Development of a High Precision Three-Dimensional Modeling System for Biological Samples

使用一个微切片图像处理系统，收集旋转刀片和自动样本的机制的因素，生物样本作为食品和内部器官已经被观察到。这就有可能观察到该系统中冰冻食品和生物材料形成的冰晶体的分布。为了观察冰晶体的分布，一种荧光染料已经被使用，而光线的最佳波浪是被选定的。以下是在前出版物文件中提出的过程和结果。冷冻食品中冰晶体形成的定量分析（日本低温生物学和低温技术学会）·冷冻干燥解决方案中冰晶体的三维度测量（日本制冷和空气调节工程师学会）·冷冻凝胶中冰晶体的非破坏性测量--利用DielectProperties对冰晶体结构的估计--（Japan Society of Refrigerating and Air conditioning Engineers）An award was given for the paper of“Non-destructive Measurement of Ice Crystals in Frozen Gelatin Gel”by Japan Society of Refrigerating and Air conditioning Engineers。A new type of Micro-Sheer图像处理系统已经提出。一台直线马达移动一把滑动刀片。这个系统不是用CCD摄像机和镜头安装的，但它使用图像扫描仪进行图像采集。在这个系统中，一个样本是切片的，切片样本的图像是自动的和连续的。与传统系统进行比较，切片样本的图像被获得了较高的准确度，因为样本是统一的，因为样本是统一的。在此之前，这个系统将变得更加紧凑，因为翻译器运动的切片刀片不需要像旋转运动那样多的空间。在获取每个切片样本的图像数据之后，三个维度图像，展示生物样本的内部信息，可以在计算机上重建。

Using a Micro-Slicer Image Processing System, which consists of a rotary blade and automatic sample feeding mechanism, biological samples such as food and internal organs have been observed. It became possible to observe the distribution of ice crystals formed in frozen foods and biological materials with this system. To observe the distribution of ice crystals a fluorescence stain was used and an optimal wavelength of light was selected. The processes and results were presented in prepublication papers shown below.・Quantitative Analysis of Ice Crystals Formed in Frozen Food (Japanese Society for Cryobiology and Cryotechnology)・Three-Dimensional Measurement of Ice Crystals in Frozen Dilute Solutions (Japan Society of Refrigerating and Air conditioning Engineers)・None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel-Estimation of Ice Crystal Structure by Utilizing Dielectric Properties-(Japan Society of Refrigerating and Air conditioning Engineers)An award was given for the paper of "None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel" by Japan Society of Refrigerating and Air conditioning Engineers.A new type of Micro-sheer image processing system was proposed. A linear motor moves a slicing blade. This system is not equipped with a CCD camera and lens, but it uses an image scanner for image acquisition. In this system, a sample is sliced and the images of sliced sample are acquired automatically and continuously. Compared with the conventional systems, the images of the sliced sample are acquired with a high degree of accuracy because the sample is illuminated uniformly. Furthermore, the system is more compact because translatory movement of a slicing blade does not need as much space as the rotary movement. After the acquisition of imge data of each sliced sample, a three dimensional image, showing the internal information of the biological sample, can be reconstructed on a computer.