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Development of a High Precision Three-Dimensional Modeling System for Biological Samples

生物样品高精度三维建模系统的开发

基本信息

批准号：
14205036
负责人：
HIGUCHI Toshiro
金额：
$ 27.96万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Using a Micro-Slicer Image Processing System, which consists of a rotary blade and automatic sample feeding mechanism, biological samples such as food and internal organs have been observed. It became possible to observe the distribution of ice crystals formed in frozen foods and biological materials with this system. To observe the distribution of ice crystals a fluorescence stain was used and an optimal wavelength of light was selected. The processes and results were presented in prepublication papers shown below.・Quantitative Analysis of Ice Crystals Formed in Frozen Food (Japanese Society for Cryobiology and Cryotechnology)・Three-Dimensional Measurement of Ice Crystals in Frozen Dilute Solutions (Japan Society of Refrigerating and Air conditioning Engineers)・None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel-Estimation of Ice Crystal Structure by Utilizing Dielectric Properties-(Japan Society of Refrigerating and Air conditioning Engineers)An award was given for the paper of "None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel" by Japan Society of Refrigerating and Air conditioning Engineers.A new type of Micro-sheer image processing system was proposed. A linear motor moves a slicing blade. This system is not equipped with a CCD camera and lens, but it uses an image scanner for image acquisition. In this system, a sample is sliced and the images of sliced sample are acquired automatically and continuously. Compared with the conventional systems, the images of the sliced sample are acquired with a high degree of accuracy because the sample is illuminated uniformly. Furthermore, the system is more compact because translatory movement of a slicing blade does not need as much space as the rotary movement. After the acquisition of imge data of each sliced sample, a three dimensional image, showing the internal information of the biological sample, can be reconstructed on a computer.

使用由旋转刀片和自动进样机构组成的显微切片图像处理系统，对食物和内脏等生物样品进行了观察。利用该系统可以观察冷冻食品和生物材料中形成的冰晶的分布。为了观察冰晶的分布，使用荧光染色并选择最佳的光波长。过程和结果在以下出版前论文中介绍。·冷冻食品中冰晶的定量分析（日本低温生物学与低温技术学会）·冷冻稀溶液中冰晶的三维测量（日本制冷空调学会）·冷冻明胶凝胶中冰晶的非破坏性测定-利用介电特性推定冰晶结构-（日本制冷空调学会）日本制冷空调学会的论文“冷冻明胶凝胶中冰晶的无损测量”获奖。提出了一种剪切图像处理系统。线性马达移动切片刀片。该系统没有配备CCD照相机和透镜，但它使用图像扫描仪进行图像采集。在该系统中，对样品进行切片，并自动连续地获取切片样品的图像。与传统系统相比，由于样品被均匀地照射，因此以高精度获取切片样品的图像。此外，该系统更紧凑，因为切片刀片的平移运动不需要与旋转运动一样多的空间。获取每个切片样本的图像数据后，可以在计算机上重建出显示生物样本内部信息的三维图像。