Immunogenetic study on Mongoloids in Asian-Pacific and Polar areas

为了从人类T细胞白血病病毒(HTLV-I)和乙肝病毒(HBV)的独特分布中阐明亚太地区蒙古族的免疫遗传学特征，本研究在北斯堪的纳维亚和俄罗斯西北部地区进行了国际比较研究。1)2003年，我们与挪威OSB大学和特罗姆斯大学的流行病学家合作，分析了挪威北部土著人群的抗HTLV-I抗体。我们在获得挪威当局和萨米人社区的正式许可后，使用了800份血清样本(400名萨米人和400名非萨米人)。所有的样本都是25年前收集的，用于其他目的，并储存在奥斯陆大学的冷冻室里。2)与阿尔汉格尔斯克大学合作，我们于2004年底针对涅涅茨自治区纳里扬马尔的涅涅茨人进行了为期三天的实地调查，我们的调查对象是涅涅茨自治区纳里扬马尔的涅涅茨人。…在征得每个受试者的知情同意后，More从Krasnoe镇的109个受试者(中心诊所的患者和普通居民)中采集了10毫升的血液样本。我们在中心诊所的实验室里从血浆中分离出了巴菲大衣，并将它们带回了阿尔汉格尔斯克大学。我们分析了HTLV-I抗体，没有找到阳性携带者。在肝炎病毒方面，我们发现3例(2.8%)抗-丙型肝炎病毒和30例(28%)抗-HBV阳性。在30例抗-HBV阳性者中，未发现HBVs抗原携带者，我们无法确定该人群中的HBVDNA基因亚型。从目前的研究，包括以前在亚洲大陆进行的研究，我们不能确定HTLV-I携带者在日本群岛以外的本土蒙古猴中的聚集性。我们需要对HTLV-I进行最终研究，以澄清蒙古人在1)格陵兰东北部因纽特人中是否存在HTLV-I，以及2)在埋葬在安第斯沙漠中8,000-500年前的木乃伊中发现HTLV-I。较少

To clarify immunogenetic characteristics of Mongoloids in Asian Pacific regions from unique distribution of human T-cell leukemia virus(HTLV-I) and hepatitis B virus(HBV), the present international comparative study was conducted in the North Scandinavian and Northwestern Russian areas.1)In cooperation with epidemiologists of Osb University and Tromse University in Norway, we analyzed anti-HTLV-I antibodies of indigenous people in North Norway in 2003. We used 800 serum samples (400 Sami people and 400 non-Sami people) after getting official permission of Norway authorities and Sami community. All samples were collected 25 years ago for the other purposes and stored in the deep freezer in Oslo University. All samples were negative anti-HTLVI and we concluded that North Norway indigenous Sami people don't carry HTLV-I.2)In cooperation with Arkhangelsk University, we conducted field work targeting Nenezt people in Nariyan Mar, Nenets autonomous areas for three days in the end of 2004 We … More collected 10 ml blood samples from 109 subjects (patients of Central Clinics and general inhabitants) in Krasnoe town after getting informed consent from each subject. We separated buffy coat from plasma in the laboratory of the Central Clinics and carried back them to Arkhangelsk University. We analyzed antibodies of HTLV-I and we could not find a positive carrier. In terms of hepatitis viruses, we found 3 (2.8%) and 30 (28%) positives of anti-HCV and anti-HBVs. Among 30 anti-HBVs positives, there is no HBV antigen carrier and we could not confirm a genetic subtype of HBV in this population.From the present study including the previous studies held in Asian Continent, we could not confirm clustering of HTLV-I carriers among indigenous Mongoloids except Japanese in Japan archipelago. We need final studies on HTLV-I to clarify Mongoloids dispersal as to 1)confirmation of HTLV-I among Inuit people in the northeastern Greenland, and 2)investigation of HTLV-I among mummies 8,000-500 years old buried in Andes desert. Less