A STUDY OF STRESS PROTEIN IN HUMAN SKELETAL MUSCLE

人类骨骼肌中应激蛋白的研究

• 批准号: 16300212
• 负责人: NAITO Hisashi
• 金额: $ 8.22万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300212/
• 关键词: Stress proteins; Heat shock protein 72; Muscle biopsy; Microwave hyperthermia; Skeletal muscle; Exercise; Delayed onset muscle soreness; ヒートショックタンパク質; 繰り返し効果; 高齢者; ハイパーサーミア; 筋温; 国際情報交換; アメリ力合衆国:トルコ共和国; HSP70; 外側広筋; アメリカ合衆国:トルコ共和国; ミオシン重鎖

It was unclear that the responses of stress protein expression to the exercise and the heat-stress in human skeletal muscles. In this study, we indicated that safe and reliable modality for inducing stress proteins in human skeletal muscle. Moreover, we also investigated the possibility of applying our results to clinical training fields. The outcome of this study was shown as follows:(1) We established the approach to research stress proteins in human skeletal muscle by using newly improved needle biopsy technique which did not disturb daily activity and training of subjects Therefore, we could develop the research field with human muscle biopsy technique.(2) It is well known that there is individual variation of stress protein expression level in sedentary condition. We revealed the stress protein expression level correlated with muscle fiber composition and strongly expressed in endurance athlete. Moreover, stress protein showed less expression level in a paralysis patient with muscle atrophy.(3) We indicated that local microwave hyperthermia treatment can increase stress proteins in human skeletal muscle. Moreover, we worked on development of the microwave hyperthermia system to provide safe and effective method to induce stress proteins in human skeletal muscle. Our method maintained the skin temperature around 40℃ and increased muscle temperature until 45℃ without muscle damage.(4) The muscle preconditioning with a microwave hyperthermia system may prevent eccentric exercise-induced muscle damage, although the effect may not apply to repeated bouts of eccentric exercise.Further investigation should examine time course of stress proteins induced by heat stress, and effect on performance in athlete.

目前尚不清楚应激蛋白表达对人类骨骼肌运动和热应激的反应。在这项研究中，我们指出了在人类骨骼肌中诱导应激蛋白的安全可靠的方式。此外，我们还研究了将我们的结果应用于临床培训领域的可能性。本研究的结果如下：(1)我们建立了利用新改进的针活检技术研究人体骨骼肌中应激蛋白的方法，该技术不干扰受试者的日常活动和训练，因此，我们可以利用人体肌肉活检技术来拓展研究领域。(2)众所周知，久坐条件下应激蛋白表达水平存在个体差异。我们揭示了应激蛋白的表达水平与肌纤维组成相关，并且在耐力运动员中强烈表达。此外，在伴有肌肉萎缩的瘫痪患者中，应激蛋白的表达水平较低。(3)我们表明，局部微波热疗可以增加人体骨骼肌中的应激蛋白。此外，我们还致力于微波热疗系统的开发，为诱导人体骨骼肌应激蛋白提供安全有效的方法。我们的方法将皮肤温度维持在40℃左右，并将肌肉温度升高至45℃，而没有肌肉损伤。（4）用微波热疗系统进行肌肉预处理可以预防离心运动引起的肌肉损伤，尽管这种效果可能不适用于重复的离心运动。进一步的研究应检查热应激诱导的应激蛋白的时间进程，以及对运动员表现的影响。