A STUDY OF STRESS PROTEIN IN HUMAN SKELETAL MUSCLE

人骨骼肌应激蛋白表达对运动和热应激的反应尚不清楚。在这项研究中，我们表明，安全可靠的方式诱导应激蛋白在人体骨骼肌。此外，我们还探讨了将我们的研究结果应用于临床培训领域的可能性。（1）建立了一种新的、不干扰受试者日常活动和训练的肌肉组织活检技术，用于研究人骨骼肌中的应激蛋白，为进一步拓展肌肉组织活检技术的研究领域奠定了基础。(2)众所周知，久坐状态下应激蛋白表达水平存在个体差异。应激蛋白的表达水平与肌纤维组成相关，在耐力运动员中有较强的表达。此外，应激蛋白在伴有肌肉萎缩的瘫痪患者中的表达水平较低。(3)我们发现局部微波热疗可以增加人体骨骼肌中的应激蛋白。此外，我们还致力于微波热疗系统的研制，为诱导人骨骼肌应激蛋白提供安全有效的方法。我们的方法使皮肤温度保持在40 ℃左右，并将肌肉温度升高至45 ℃，而不损伤肌肉。(4)微波热疗系统的肌肉预处理可以防止离心运动引起的肌肉损伤，尽管这种效果可能不适用于重复的离心运动。进一步的研究应该检查热应激诱导的应激蛋白的时间过程，以及对运动员成绩的影响。

It was unclear that the responses of stress protein expression to the exercise and the heat-stress in human skeletal muscles. In this study, we indicated that safe and reliable modality for inducing stress proteins in human skeletal muscle. Moreover, we also investigated the possibility of applying our results to clinical training fields. The outcome of this study was shown as follows:(1) We established the approach to research stress proteins in human skeletal muscle by using newly improved needle biopsy technique which did not disturb daily activity and training of subjects Therefore, we could develop the research field with human muscle biopsy technique.(2) It is well known that there is individual variation of stress protein expression level in sedentary condition. We revealed the stress protein expression level correlated with muscle fiber composition and strongly expressed in endurance athlete. Moreover, stress protein showed less expression level in a paralysis patient with muscle atrophy.(3) We indicated that local microwave hyperthermia treatment can increase stress proteins in human skeletal muscle. Moreover, we worked on development of the microwave hyperthermia system to provide safe and effective method to induce stress proteins in human skeletal muscle. Our method maintained the skin temperature around 40℃ and increased muscle temperature until 45℃ without muscle damage.(4) The muscle preconditioning with a microwave hyperthermia system may prevent eccentric exercise-induced muscle damage, although the effect may not apply to repeated bouts of eccentric exercise.Further investigation should examine time course of stress proteins induced by heat stress, and effect on performance in athlete.