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The study of infectious diseases in infants and children in Asia

亚洲婴儿和儿童传染病研究

基本信息

批准号：
16406030
负责人：
USHIJIMA Hiroshi
金额：
$ 6.4万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Development of diagnosis and molecular epidemiological study have done in Asian children mainly with viral gastroenteritis, viral hepatitis and HIV infection. (1) As diagnosis, we newly developed immunochromatography and LAMP for norovirus detection. Real time PCR was used for low amount of norovirus RNA for environment. In addition, we developed multiplex PCR for screening of diarrheal viruses in stool specimens from Thailand, China, Vietnam, Bangladesh, Srilanka and Eastern Russia and for HIV subtypes in blood samples. (2) As molecular epidemiology, rotavirus, enteric adenovirus, astrovirus, sapovirus and norovirus were examined. Rate of rotavirus infection is higher in Asian countries than in Japan. While, rate of norovirus infection is higher in Japan than in Asian countries. Generally, G1 rotavirus decreased and G9 as well as G3 sequentially increased from 2000, however, G1 rotavirus increased again after 2006. Lineage in each genotype changed year by year. Reassortant rotaviruses were found in human and porcine. New P[27] genotype was discovered in porcine. Genotype 41 enteric adenovirus has still been main type. Genotypes 40 and 41 of adenovirus were divided into subtypes and new subtype was found. Norovirus GII/4 was major type in all countries. New recombinant norovirus appeared and then it became the major type every year. Different norovirus clusters were found sometimes in year and country, however generally almost same cluster of norovirus appeared simultaneously in all countries. Genotype 1 and subtype C were major in astrovirus and hepatitis B viruses, respectively. Gastroenteritis viruses were examined in imported oyster from China for 1 year. Norovirus was found mostly but a small number of rotavirus was also found in oysters. Of these GII/4 and GII/3 noroviruses were predominant genotypes.

亚洲儿童主要是病毒性胃肠炎、病毒性肝炎和HIV感染者，其诊断和分子流行病学研究取得了进展。(1)作为诊断，我们新开发的免疫层析和LAMP检测诺如病毒。真实的时间PCR用于环境中低量的诺如病毒RNA。此外，我们还开发了多重PCR技术，用于筛查来自泰国、中国、越南、孟加拉国、斯里兰卡和俄罗斯东部的粪便标本中的埃博拉病毒，以及血液样本中的HIV亚型。(2)作为分子流行病学，检测了轮状病毒、肠道腺病毒、星状病毒、沙波病毒和诺如病毒。亚洲国家的轮状病毒感染率高于日本。而日本的诺如病毒感染率高于亚洲国家。从2000年开始，G1轮状病毒总体呈下降趋势，G9和G3轮状病毒依次上升，但2006年后G1轮状病毒再次上升。各基因型的谱系逐年变化。在人和猪中均发现了耐药轮状病毒。在猪中发现了新的P[27]基因型。基因型41肠道腺病毒仍是主要类型。基因型40和41型腺病毒进行了亚型划分，并发现了新的亚型，诺如病毒GII/4型是各国的主要亚型。新的重组诺如病毒不断出现，并成为每年的主要类型。诺如病毒在不同年份和国家间有时会出现不同的病毒簇，但一般而言，几乎同一病毒簇在所有国家同时出现。星状病毒以基因1型为主，B型肝炎病毒以C亚型为主。对从中国进口的牡蛎进行了为期1年的胃肠炎病毒检测。在牡蛎中发现的病毒主要是诺如病毒，但也有少量轮状病毒。其中诺如病毒GII/4和GII/3基因型占优势。

项目成果

期刊论文数量（138）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Changing Distribution of Norovirus Genotypes and Genetic Characterization of Recombinant GIIb among Infants and Children with Diarrhea in Japan

日本腹泻婴儿和儿童中诺如病毒基因型分布的变化和重组 GIIb 的遗传特征

DOI：
发表时间：
2006
期刊：
J Med Virol 78
影响因子：
0
作者：
Sugawara K.;Tajima Y.;Kawashima I.;Tsukimura T.;Saito S.;Ohno K.;Iwamoto K.;Kobayashi T.;Itoh K.;Sakuraba H.;Phan TG
通讯作者：
Phan TG

Emergence of new variant rotavirus G3 among infants and children with acute gastroenteritis in Japan during 2003-2004.

DOI：
发表时间：
2007
期刊：
Clinical laboratory
影响因子：
0.7
作者：
T. Phan;Q. D. Trinh;P. Khamrin;K. Kaneshi;Y. Ueda;S. Nakaya;S. Nishimura;K. Sugita;T. Nishimura;A. Yamamoto;S. Takanashi;F. Yagyu;S. Okitsu;H. Ushijima
通讯作者：
T. Phan;Q. D. Trinh;P. Khamrin;K. Kaneshi;Y. Ueda;S. Nakaya;S. Nishimura;K. Sugita;T. Nishimura;A. Yamamoto;S. Takanashi;F. Yagyu;S. Okitsu;H. Ushijima

Existence of multiple outbreaks of viral gastroenteritis among infants in a day care center in Japan