The study of the development of diagnostic methods, molecular epidemiology and risk assessment for viral infections diseases.

1)以诺如病毒样颗粒为抗原制备诺如病毒多克隆抗体和单克隆抗体。研制并检测了在世界上广泛传播的诺如病毒GII/3和GII/4免疫层析检测试剂盒。该试剂盒具有临床应用价值，检测结果与RT-PCR结果一致。我们目前正在开发新的试剂盒，适用于几乎所有基因型的诺如病毒，包括基因组i。2)我们评估了LAMP法检测诺如病毒。低剂量诺如病毒RNA检测方便，但新的GI基因型引物有待进一步评价。3)获得了一种诺如病毒单克隆抗体，对胃肠道和胃肠道诺如病毒有广泛的反应。单克隆抗体在构象上识别诺如病毒。我们首先报告了识别地点。4)我们不仅在腹泻患儿的粪便样本中检测到诺如病毒RNA，而且在腹泻患儿的血清样本中也检测到诺如病毒RNA。一些样本来源于有神经学发现的病人。5)开发了新的牡蛎轮状病毒浓缩提取方法。我们在临床样本中使用了它。6)对2004-2005年（第一期）、2005-2006年（第二期）和2006-2007年（第三期）日本婴幼儿胃肠炎病原病毒进行了检测。第1期以诺如病毒为主，第2、3期以轮状病毒为主。轮状病毒1期以G3轮状病毒为主，2期以G1轮状病毒为主。在我们的研究中也发现了G3轮状病毒变体。诺如病毒GII/4在第一期为优势病毒，是一种新的重组病毒。GII/3诺如病毒为第二阶段的优势病毒，也是一种新的重组病毒。在第一期发现了GI/1腺病毒，它也是一种新的重组病毒。7)比较不同临床严重程度患者巨细胞病毒分子流行病学资料。所有基因型的gB、UL144和UL149与先天性和获得性巨细胞病毒感染的神经系统表现均相关。然而，gB3在先天性巨细胞病毒感染中很常见。少

1)Norovirus polyclonal and monoclonal antibodies were produced by using norovirus-like particles as antigen. Immunoclomatography test kit for norovirus GII/3 and GII/4 which spread widely in the world was developed and tested. The kit was useful clinically and the results were compatible to those of RT-PCR. We are now developing new kit which is preferable to almost all genotypes of norovirus including genogroup I.2)We evaluated LAMP method for norovirus. It was convenient to detect low amount of norovirus RNA, but new primers for GI genotype need to be further evaluate.3)We obtained a norovirus monoclonal antibody which reacts broadly against GI and GII noroviruses. The monoclonal antibody recognized noroviruses conformationally. We reported the recognition site firstly.4)We detected norovirus RNA not only from stool samples but also from serum samples of diarrheal children. Some samples derived from the patients with neurological findings.5)We developed new concentration and extracti … More on method of rotavirus from oyster. We used it in clinical samples.6)We examined the causative viruses of gastroenteritis in Japanese infants in 2004-2005 (1st Period), 2005-2006 (2nd Period) and 2006-2007 (3rd Period). Norovirus was the most predominant virus in the 1st period, but rotavirus was predominant in the 2nd and 3rd periods. For rotavirus study, G3 rotavirus was predominant in the 1st period but G1 rotavirus was predominant in 2nd period. The G3 rotavirus variants was also identified in our study. Norovirus GII/4 was predominant in 1st period and it was identified as a new recombinant virus. GII/3 norovirus was predominant in 2nd period and it also a new recombinant viruses. GI/1 sapovirus was found in the 1st period and it was also new recombinant virus.7)We compared the molecular epidemiological data of cytomegalovirus from patients with the clinical severity. All genotypes of gB, UL144 and UL149 equally related to neurological manifestations in congenital and acquired cytomegalovirus infection. However, gB3 was common in congenital cytomegalovirus infection. Less