The study of the development of diagnostic methods, molecular epidemiology and risk assessment for viral infections diseases.

研究病毒感染疾病的诊断方法、分子流行病学和风险评估的发展。

• 批准号: 18390297
• 负责人: USHIJIMA Hiroshi
• 金额: $ 11.02万
• 依托单位: The International University of Kagoshima (2007)The University of Tokyo (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390297/
• 关键词: virus; infectious disease; diagnosis; epidemiology; pathophysiology; ウイルス性下痢症; ノロウイルス; ロタウイルス; イムノクロマトグラフィー; LAMP法; RT-PCR法

1)Norovirus polyclonal and monoclonal antibodies were produced by using norovirus-like particles as antigen. Immunoclomatography test kit for norovirus GII/3 and GII/4 which spread widely in the world was developed and tested. The kit was useful clinically and the results were compatible to those of RT-PCR. We are now developing new kit which is preferable to almost all genotypes of norovirus including genogroup I.2)We evaluated LAMP method for norovirus. It was convenient to detect low amount of norovirus RNA, but new primers for GI genotype need to be further evaluate.3)We obtained a norovirus monoclonal antibody which reacts broadly against GI and GII noroviruses. The monoclonal antibody recognized noroviruses conformationally. We reported the recognition site firstly.4)We detected norovirus RNA not only from stool samples but also from serum samples of diarrheal children. Some samples derived from the patients with neurological findings.5)We developed new concentration and extracti … More on method of rotavirus from oyster. We used it in clinical samples.6)We examined the causative viruses of gastroenteritis in Japanese infants in 2004-2005 (1st Period), 2005-2006 (2nd Period) and 2006-2007 (3rd Period). Norovirus was the most predominant virus in the 1st period, but rotavirus was predominant in the 2nd and 3rd periods. For rotavirus study, G3 rotavirus was predominant in the 1st period but G1 rotavirus was predominant in 2nd period. The G3 rotavirus variants was also identified in our study. Norovirus GII/4 was predominant in 1st period and it was identified as a new recombinant virus. GII/3 norovirus was predominant in 2nd period and it also a new recombinant viruses. GI/1 sapovirus was found in the 1st period and it was also new recombinant virus.7)We compared the molecular epidemiological data of cytomegalovirus from patients with the clinical severity. All genotypes of gB, UL144 and UL149 equally related to neurological manifestations in congenital and acquired cytomegalovirus infection. However, gB3 was common in congenital cytomegalovirus infection. Less

1)以诺如病毒样颗粒为抗原，制备诺如病毒多克隆抗体和单抗。研制了国际上流行的诺如病毒GII/3和GII/4免疫层析检测试剂盒，并进行了检测。该试剂盒具有较高的临床实用价值，检测结果与RT-PCR结果一致。我们正在开发新的试剂盒，该试剂盒适用于几乎所有诺沃克病毒的基因类型，包括I基因组。2)我们对LAMP方法进行了评估。该方法检测诺如病毒RNA含量较低，但针对Gi基因的新引物尚需进一步研究。3)获得了一株能与Gi和Gii诺如病毒发生广泛反应的诺如病毒单抗。该抗体可识别诺如病毒的构象。4)我们不仅从粪便样本中检测到诺如病毒RNA，还从腹泻儿童的血清样本中检测到诺如病毒RNA。5)我们开发了新的浓缩和提取…更多关于牡蛎轮状病毒的方法。6)我们检测了2004-2005年(第一期)、2005-2006年(第二期)和2006-2007年(第三期)日本婴儿胃肠炎的致病病毒。第1期以诺如病毒为主，第2、3期以轮状病毒为主。轮状病毒研究第1期以G3轮状病毒为主，第2期以G1轮状病毒为主。G3轮状病毒变异体也在我们的研究中被发现。前期以诺如病毒GII/4为主，经鉴定为一种新的重组病毒。第二期以GII/3诺如病毒为主，是一种新的重组病毒。在第1期发现GI/1病毒，也是新的重组病毒。7)比较患者巨细胞病毒分子流行病学资料与临床严重程度。在先天性和获得性巨细胞病毒感染中，GB、UL144和UL149三种基因型与神经系统症状的相关性相同。然而，Gb3在先天性巨细胞病毒感染中很常见。较少

项目成果

Development and validation of DNA microarray for genotyping group A rotavirus VP4(P[4]P[6],P[8],P[9]andP[14]) and VP7 (G1-G6,G8-G10and G12)genes

用于 A 组轮状病毒 VP4（P[4]P[6]、P[8]、P[9] 和 P[14]）和 VP7（G1-G6、G8-G10 和 G12）基因基因分型的 DNA 微阵列的开发和验证

• 发表时间: 2007
• 期刊: J Clin Microbiol 45
• 作者: 青木洋子;松原洋一;Honma S
• 通讯作者: Honma S

Outbreak of sapovirus infection among infection among infants and children with acutegastroenteritis in Osaka City,Japan during during 2004-2005

2004-2005年日本大阪市婴幼儿急性胃肠炎感染中沙波病毒感染暴发情况

• 发表时间: 2006
• 期刊: J Med Virol 78
• 作者: Kawashima I.;Watabe K.;Tajima Y.;Fukushima T.;Kanzaki T.;Kanekura T.;Sugawara K.;Ohyanagi N.;Suzuki T.;Togawa T.;Sakuraba H.;平家俊男;Phan TG
• 通讯作者: Phan TG

Molecular characterization of a rare G3P[3] human rotavirus reassortant strain reveals an evidence for human-animals multip interspecies transmissions

一种罕见的 G3P[3] 人轮状病毒重配株的分子特征揭示了人与动物多种种间传播的证据

• 发表时间: 2006
• 期刊: J Med Virol 78(7)
• 作者: Khamrin P;et al.
• 通讯作者: et al.

沖縄県中部の2006年9月の-市中病院のノロウイルス集団感染における迅速診断イムノクロマト(NVIC-1)の有用性の検討

2006 年 9 月冲绳县中部一家市立医院诺如病毒爆发中快速诊断免疫色谱法 (NVIC-1) 的有效性检查

• 发表时间: 2007
• 作者: Kimura S;Tateda K;Ishii Y;Horikawa M;Miyairi S;Gotoh N;Ishiguro M;Yamaguchi K.;石田 眞一
• 通讯作者: 石田 眞一

シュードプロテオグリカン(シュードPG)のリガンド結合性と抗HIV-1活性

假蛋白多糖 (pseudoPGs) 的配体结合和抗 HIV-1 活性

• 发表时间: 2007
• 作者: Kai T;Tateda K;Kimura S;Ishii Y;Ito H;Yoshida H;Kimura T;Yamaguchi K.;中村 公亮
• 通讯作者: 中村 公亮