Basic and clinical research of viral gastroenteritis in children and its risk evaluation

我们已经生产了新的诺沃克病毒和萨普病毒的病毒样颗粒(VLP)，以更新病毒遗传变异的后果。这些新的VLP已经开发/改进了免疫层析(IC)诊断试剂盒。除了我们所做的轮状病毒、诺如病毒、SAP病毒、腺病毒和星状病毒的分子流行病学研究外，在腹泻粪便中还发现了新的腹泻病毒，如Aichi病毒、ParechoVirus、BocaVirus和Saffold病毒。用小鼠诺如病毒作为一种新的替代物来评价消毒剂、加热和抗病毒活性。我们挑战了诺沃克病毒的多细胞培养试验，但目前没有成功。我们还检测了诺如病毒和皂病毒与唾液和肠道细胞的组织血型抗原的结合活性。有趣的是，发现诺沃克病毒，而不是沙巴病毒，显示出高结合活性。

We have produced new virus-like particles(VLPs) of norovirus and sapovirus in order to update the consequences of viral genetic variability. The immunochromatography(IC) diagnosis test kits have been developed/improved by these new VLPs. In addition to the molecular epidemiological study of rotavirus, norovirus, sapovirus, adenovirus and astrovirus that we have done, the novel diarrheal viruses such as Aichi virus, parechovirus, bocavirus and Saffold virus have also been found in diarrheal stool samples. Murine norovirus was used as a novel surrogate to evaluate disinfectants, heating, and antiviral activity for human norovirus. We challenged multiple-cell cultivation assays for norovirus but did not succeed at this moment. We also examined the binding activities of norovirus and sapovirus to histo-blood group antigens of saliva and intestinal cells. It was interesting to find that norovirus, but not sapovirus, showed high binding activity.