Studies on control mechanisms of cytoskeletal networks maintaining the morphology of mammalian eggs

1. 短句来源利用荧光成像技术研究了MPF、MAPK和Rho激酶对仓鼠卵中角蛋白分布的影响。本研究结果表明，在卵母细胞成熟过程中，角蛋白在细胞质中存在，其分布动态变化，从GV期的集中分布到M II期的纤维状分布。当MPF和MAP激酶被抑制时，角蛋白的分布未发生变化。另一方面，当Rho激酶被抑制时，没有明显的变化。这表明角蛋白在哺乳动物卵中的分布可能受MPF和MAP激酶的控制，而不受Rho激酶的控制。Rho激酶在猪卵母细胞成熟过程中的功能作用研究了Rho激酶在猪卵母细胞成熟过程中的作用。本研究表明，当Rho激酶被抑制时，生发囊泡（GV）的破坏和第一极体的排出受到抑制。然而，染色体的分离不受影响。此外，即使在成熟孵育后18小时阻断Rho激酶，也会发生GV分解。结果表明，Rho激酶在培养18 h时引起GV分解的调控机制中起一定作用。荧光观察显示，Rho激酶抑制剂使卵母细胞内微丝密度明显下降，导致第一极体未从卵母细胞中释放出来。经Rho激酶抑制剂孵育的卵母细胞-卵丘细胞复合物在成熟44小时后未见卵丘细胞扩增。除了微丝的结构外，还提出了影响微管在积云细胞中分布的可能性。

1. Functional roles of cell-cycle control kinase on the keratin distribution of the mammal eggs Influences of MPF, MAPK and Rho kinase on the distribution of keratin in the hamster eggs were investigated by the fluorescent-imaging. The present results clarified that hamster eggs included keratin in the cytoplasm, of which distribution changed dynamically from the concentrated pattern at the GV stage into the fibrous pattern at the M II stage during oocyte maturation. When MPF and MAP kinase were inhibited, the distributional change of the keratin had not happened. On the other hand, no remarkable changes was seen when Rho kinase was inhibited. It is suggested that the distribution of keratin in the mammal eggs may be controlled by MPF and MAP kinase, but not by Rho kinase.2. Functional role of Rho kinase in maturation process of the pig oocytes Functional role of Rho kinase during oocyte maturation was studied in the pig. The present study revealed that the germinal-vesicle (GV) breakdown and expulsion of the first polar body was restrained when Rho kinase was inhibited. However, the separation of the chromosome wasn't affected. In addition, the GV breakdown had occurred even if Rho kinase was blocked 18 hours after maturation incubation. It is suggested that Rho kinase may play a role in the control mechanism which causes GV breakdown by 18 hours of cultivation. Fluorescent observations revealed that the microfilament in the oocytes declined remarkably in density by Rho kinase inhibitor, thereby the first polar body was not released from the oocytes. The oocytes-cumulus cells complexes incubated with Rho kinase inhibitor didn't show the cumulus cell expansion after 44 hr of maturation. The possibility affecting the distribution of microtubules among the cumulus-cells was suggested in addition to the architecture of microfilaments.