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Functional analysis and identification of substrate proteins of mitochondrial sirtuins.

Functional analysis and identification of substrate proteins of mitochondrial sirtuins.
线粒体去乙酰化酶底物蛋白的功能分析和鉴定。
批准号：
20770107
负责人：
SUZUKI Hiroyuki
金额：
$2.75万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2008
资助国家：
日本
项目状态：
已结题
起止时间：
2008 至 2009
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770107/
关键词：
酵素の調節タンパク質修飾細胞小器官酵素発現制御老化

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英文摘要
Recently the target proteins of mitochondrial sirtuins (SIRT3,4,5) were clarified respectively, still a lot of things are the mysteries for the function of mitochondrial sirtuins. To investigate the role of the mitochondrial sirtuins, we have comprehensively disrupted the gene of mitochondrial sirtuins using DT40 cells. We succeeded in single sirtuin-deficient cells and double sirtuin-deficient cells by present. The little delay was seen in growth in double sirtuin-deficient cells compared with wild type cells though these deficient cells were able to grow everything.
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