Physiomic analyses of cardiac hypertrophy and ventricular geometry of in cardiac regulation

心泵功能是由心肌和心室几何形状的数量和质量决定的。我们研究了心脏对环境变化的调节机制。首先，我们聚焦于围生期氧张力变化对细胞从分裂到肥厚转换的影响。采集胎儿心肌细胞，在不同氧张力（低氧：3%，高氧：20%）下培养。我们首次成功地利用延时动画技术对低氧条件下心肌细胞的分裂过程进行了可视化。然后，我们发现心肌细胞在高氧条件下停止细胞分裂，而在低氧条件下继续分裂。我们还进行了微缓速分析，以揭示细胞周期调控基因，这将有助于使成人心肌细胞重新开始细胞分裂。至于心脏的机制感知，我们发现TRPV2阳离子通道对心脏结构和功能的维持至关重要。

Cardiac pump function is determined by the quantity and quality of myocardium and ventricular geometry. We investigated the regulatory mechanism of the heart for the change in environment. First, we focused the effects of the change in oxygen tension on the cellular switching from division to hypertrophy during perinatal period. Fetal cardiomyocytes were harvested and cultured at different oxygen tension (low O2: 3%, high O2: 20%) . We firstly succeeded to visualize the division of cardiomyocytes using time-lapse motion picture under low O2 condition. Then, we found that cardiomyocytes stopped cell division under high O2 while they kept dividing under low O2 condition. We also performed micro-allay analysis to reveal the genes regulating cell cycle, which would be useful to make the adult cardiomyocytes to restart cell devision. As for the mechan-osensing in the heart, we show that TRPV2 cation channel is critical for the maintenance of cardiac structure and function.