Physiomic analyses of cardiac hypertrophy and ventricular geometry of in cardiac regulation

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Cardiac pump function is determined by the quantity and quality of myocardium and ventricular geometry. We investigated the regulatory mechanism of the heart for the change in environment. First, we focused the effects of the change in oxygen tension on the cellular switching from division to hypertrophy during perinatal period. Fetal cardiomyocytes were harvested and cultured at different oxygen tension (low O2: 3%, high O2: 20%) . We firstly succeeded to visualize the division of cardiomyocytes using time-lapse motion picture under low O2 condition. Then, we found that cardiomyocytes stopped cell division under high O2 while they kept dividing under low O2 condition. We also performed micro-allay analysis to reveal the genes regulating cell cycle, which would be useful to make the adult cardiomyocytes to restart cell devision. As for the mechan-osensing in the heart, we show that TRPV2 cation channel is critical for the maintenance of cardiac structure and function.