Physiomic analyses of cardiac hypertrophy and ventricular geometry of in cardiac regulation

心脏肥大和心室几何形状的生理学分析

• 批准号: 23300171
• 负责人: MOHRI Satoshi
• 金额: $ 11.23万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300171/
• 关键词: 心機能; 心肥大; 心筋分裂; 酸素環境; 機械受容; 冠循環; フィジオーム; カルシウム; 心不全

Cardiac pump function is determined by the quantity and quality of myocardium and ventricular geometry. We investigated the regulatory mechanism of the heart for the change in environment. First, we focused the effects of the change in oxygen tension on the cellular switching from division to hypertrophy during perinatal period. Fetal cardiomyocytes were harvested and cultured at different oxygen tension (low O2: 3%, high O2: 20%) . We firstly succeeded to visualize the division of cardiomyocytes using time-lapse motion picture under low O2 condition. Then, we found that cardiomyocytes stopped cell division under high O2 while they kept dividing under low O2 condition. We also performed micro-allay analysis to reveal the genes regulating cell cycle, which would be useful to make the adult cardiomyocytes to restart cell devision. As for the mechan-osensing in the heart, we show that TRPV2 cation channel is critical for the maintenance of cardiac structure and function.

心脏泵功能由心肌的数量和质量以及心室几何形状决定。我们探讨了心脏对环境变化的调节机制。 首先，我们重点研究了围产期氧张力变化对细胞从分裂到肥大转变的影响。取胎鼠心肌细胞，在不同氧分压（低氧：3%，高氧：20%）下培养。我们首次成功地在低氧条件下使用延时运动图像可视化心肌细胞的分裂。然后，我们发现心肌细胞在高O2条件下停止细胞分裂，而在低O2条件下继续分裂。我们还进行了微阵列分析，以揭示细胞周期调控基因，这将有助于使成年心肌细胞重新开始细胞分裂。对于心脏的机械感受，我们发现TRPV2阳离子通道对于维持心脏结构和功能至关重要。

项目成果

TRPV2 is critical for the maintenance of cardiac structure and function in mice.

• DOI: 10.1038/ncomms4932
• 发表时间: 2014-05-29
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Katanosaka, Yuki;Iwasaki, Keiichiro;Ujihara, Yoshihiro;Takatsu, Satomi;Nishitsuji, Koki;Kanagawa, Motoi;Sudo, Atsushi;Toda, Tatsushi;Katanosaka, Kimiaki;Mohri, Satoshi;Naruse, Keiji
• 通讯作者: Naruse, Keiji

Effects of propofol on left ventricular mechanoenergetics in the excised cross-circulated canine heart

异丙酚对离体交叉循环犬心脏左心室机械能学的影响

• 发表时间: 2012
• 期刊: Acta Med Okayama
• 影响因子: 0.5
• 作者: Fujinaka W;Shimizu J;Iribe G;Imaoka T;Oshima Y;Kiyooka T;Morita K;Mohri S
• 通讯作者: Mohri S