Changes in blood cytokine levels under stress conditions and their application to forensic diagnosis.

研究的细胞因子有IFN-γ、IL-6、IL-10、IL-12p70、MCP-1和TNF。首先，我们利用Pq中毒小鼠模型研究了百草枯（Pq）对细胞因子水平的影响。结果表明，Pq暴露后5 d内，组织中IL-6和MCP-1 mRNA水平和血液中蛋白质水平均显著升高。前者可能是肺急性损伤的早期标志，后者可能是肺急性损伤的晚期标志。现研究抗IL-6抗体对pq中毒损伤的作用，有待进一步研究。接下来，我们使用水浸约束应激（WRS）小鼠模型研究了甲基苯丙胺（MA）对细胞因子水平的影响。应激条件下血清细胞因子IL-6、IL-10、TNF水平升高，应激条件下MA显著升高；特别是IL-6水平协同升高。另一方面，MA对应激性胃损伤有明显的抑制作用，但其机制有待进一步研究。我们利用ICR小鼠研究了GR多态性在应激反应中的作用。我们的研究结果表明，GR基因多态性对应激诱导的输出有显著影响，包括细胞因子、皮质酮、血清Hsp70水平和胃病变指数。这些研究为GR在个体应激反应中的作用提供了见解。

The cytokines investigated were IFN-γ, IL-6, IL-10, IL-12p70, MCP-1 and TNF. First, we investigated the effect of paraquat (Pq) on cytokine levels using the model of Pq-poisoned mice. As the result, IL-6 and MCP-1 levels, both mRNA in tissues and protein levels in blood, significantly increased within 5 days after Pq exposure. The former may be an early marker and the latter be a late marker for lung acute damage. Now we examine the effect of anti IL-6 antibody to Pq-poisoned damage, which need further investigation. Next, we examined the effects of methamphetamine (MA) on cytokine levels using a water-immersion restraint stress (WRS) mouse model. The levels of the serum cytokines IL-6, IL-10 and TNF were increased by stress, and were markedly increased by MA under stress conditions ; in particular, the level of IL-6 was synergistically increased. On the other hand, MA resulted in significant suppression of the stress-induced gastric injury, although further studies are necessary to resolve the mechanism. We investigated the involvement of the GR polymorphism in stress response using ICR mice. Our results suggest that the GR gene polymorphism has a significant impact on the stress-induced output, including cytokines, corticosterone, Hsp70 levels in serum, and the gastric lesion index. These studies provide insights into the role of GR in individual responses to stress.