A novel biotechnology of gene therapy and human embryonic stem cells for regenerative medicine

用于再生医学的基因治疗和人类胚胎干细胞的新型生物技术

• 批准号: 19790547
• 负责人: TAKAHASHI Tomoyuki
• 金额: $ 2.36万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19790547/
• 关键词: 心臓病態学; ヒトES細胞; 心筋発生・分化; 再生療法; 心筋分化・発生

これまで申請者は、胚性幹(ES)細胞による難治性循環器疾患の再生医療技術開発を目指したマウスES細胞における心筋分化誘導法(Kawai-T, Takahashi-T, et al. '04Circ J.)、ならびにES細胞由来心筋分化細胞の同定・単離技術の開発(Adenoviral conditionaltargeting法)を行ってきた(Takahashi-T, et al. '06 Mol. Ther.)。本研究課題では、マウスES細胞研究において培ってきた分化誘導法や目的細胞の同定・単離技術をヒトES細胞において応用する事によって、主にアデノウイルスベクターを利用したヒトES細胞による再生医療技術開発を行なった。

The technique of regenerative medicine for the treatment of circulatory diseases (Kawai-T, Takahashi-T, et al) aims to treat the disease of ES patients. 04Circ J.), the origin and differentiation of ES cells are the same as those of the Adenoviral conditionaltargeting method (Takahashi-T, et al.) '06 Mol. Ther.). The purpose of this study is to determine the effectiveness of the method of differentiation and differentiation of ES cells in this study. in this study, the purpose of this study is to determine the same purpose of this study. The purpose of this study is to establish the same method for the development of regenerative medicine. The purpose of this study is to use the technology of regenerative medicine to improve the performance of regenerative medicine. In this study, the purpose of this study is to use the technology of regenerative medicine.

项目成果

Conditional replicating adenovirus regulated by SURVIVIN and CER promoters exerted more strict cancer specificity and potent anti-cancer effect

SURVIVIN和CER启动子调控的条件复制腺病毒具有更严格的癌症特异性和强大的抗癌作用

• 发表时间: 2007
• 作者: Murofushi Y.;Kamizono J.;Khai NC.;Takahashi T.;Komiya S.,and Kosai K.
• 通讯作者: Komiya S.,and Kosai K.

川崎病急性期における流血中の樹状細胞の動態Significant decrease of circulating myeloid dendritic cells in acute phase of Kawasaki disease

川崎病急性期循环骨髓树突状细胞显着减少

• 发表时间: 2008
• 作者: 須田憲治;安川秀雄;高橋知之;岡村尚昌;西野裕;横山隆人;工藤嘉公;石井治佳;家村素史;松石豊次郎
• 通讯作者: 松石豊次郎

Application of an adenoviral vector encoding soluble transforming growth factor-beta type II receptor to the treatment of diabetic nephropathy in mice

编码可溶性转化生长因子-βII型受体的腺病毒载体在治疗小鼠糖尿病肾病中的应用

• 发表时间: 2008
• 期刊: Clin Exp Pharmacol Physiol 35(11)
• 作者: Kondo T.;Takemura G.;Kosai K.;Ohno T.;Takahashi T.;Esaki M.,Goto K.;Maruyama R.;Murata I.;Minatoguchi S.;Fujiwara T.;Fujiwara H.
• 通讯作者: Fujiwara H.

Combined therapy with cardioprotective cytokine administration and antiapoptotic gene transfer in postinfarction heart failure

• DOI: 10.1152/ajpheart.01147.2008
• 发表时间: 2009-03-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
• 影响因子: 4.8
• 作者: Okada, Hideshi;Takemura, Genzou;Minatoguchi, Shinya
• 通讯作者: Minatoguchi, Shinya

In vivo hepatocyte growth factor gene transfer reduces myocardial ischemia-reperfusion injury through its multiple actions

• DOI: 10.1016/j.cardfail.2007.07.004
• 发表时间: 2007-12-01
• 期刊: JOURNAL OF CARDIAC FAILURE
• 影响因子: 6
• 作者: Chen, Xue-Hai;Minatoguchi, Shinya;Fujiwara, Hisayoshi
• 通讯作者: Fujiwara, Hisayoshi