Development of CGRP-gene-transfected immune regulatory cells for treatment of refractory uveoretinitis in human

开发CGRP基因转染的免疫调节细胞用于治疗人类难治性葡萄膜视网膜炎

• 批准号: 19592041
• 负责人: KEZUKA Takeshi
• 金额: $ 2.58万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592041/
• 关键词: CGRP; 遺伝子導入細胞; 難治性ぶどう膜炎; 制御性細胞; 神経ペプチド; 難治性視神経炎; ぶどう膜炎; 遺伝子治療; 樹状細胞

難治性ぶどう膜炎患者由来樹状細胞を用いた神経ペプチド (CGRP) 遺伝子の導入と、その免疫学的解析を行った。健常者の単核球同様、CGRP 遺伝子導入細胞からのCGRP 産生は40-60pg/mlであった。難治性ぶどう膜炎を持つベーチェット病患者から得られたCGRP 遺伝子導入細胞由来の培養上清中サイトカインは、対照群と比べて有意差を見いだせなかった。CGRP 刺激樹状細胞表面分子で免疫調節に関与する接着分子を検討したが、有意な結果が得られなかった。これらのことから、正常細胞を用いたCGRP 遺伝子導入実験では、制御性細胞を作成できるが、ベーチェット病患者由来の細胞では免疫制御細胞を誘導しなかった。今後、他の難治性ぶどう膜炎でも同様に検討する必要がある。

The etiology of the patients with idiopathic inflammatory disease was analyzed by the methods of immunological analysis and immunological analysis. The etiology of the patients with chronic inflammatory disease was analyzed by the method of immunological analysis. The normal patients had the same nuclear ball, and the CGRP had the same nuclei. The normal CGRP gave birth to 40-60pg/ml cells. The cause of the disease was that the patients were infected with CGRP, and the supernatant of the supernatant was better than that of the patients. CGRP stimulated the immune response of the molecules on the surface of the cells, followed by the immune response of the molecules and the intentional results. The normal cells are infected with CGRP cells, and the immune system cells are used to guide the immune system of patients with disease. From now on, he will treat the disease with the same symptoms as necessary.

项目成果

Angiotensin II type 1 receptor blocker telmisartan suppresses experimental autoimmune uveitis

血管紧张素 II 1 型受体阻滞剂替米沙坦抑制实验性自身免疫性葡萄膜炎

• 发表时间: 2009
• 期刊: Invest Ophthalmol Vis Sci (in press)
• 作者: Okunuki Y;Usui Y;Nagai N;Kezuka T;Ishida S;Takeuchi M;Goto H
• 通讯作者: Goto H

Increased levels of monokine induced by interferon-gamma (Mig) in the vitreous of patients with diabetic retinopathy.

糖尿病视网膜病变患者玻璃体中干扰素-γ (Mig) 诱导的单核因子水平升高。

• 发表时间: 2008
• 期刊: Diabet Med. 25
• 作者: Okunuki;Y;Saika S;Wakabayashi Y
• 通讯作者: Wakabayashi Y

Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens

• 发表时间: 2008-06
• 期刊: Molecular Vision
• 影响因子: 2.2
• 作者: Y. Okunuki;Y. Usui;T. Kezuka;T. Hattori;K. Masuko;Hiroshi Nakamura;K. Yudoh;H. Goto;M. Usui;K. Nishioka;Tomohiro Kato;M. Takeuchi

Gene therapy with calcitonin gene-related peptide suppresses murine experimental autoimmune uveoretinitis

降钙素基因相关肽的基因治疗可抑制小鼠实验性自身免疫性葡萄膜视网膜炎

• 发表时间: 2008
• 作者: Kezuka;T. et al
• 通讯作者: T. et al

Proteomic surveillance of autoimmunity in Behcet's disease with uveitis : selenium binding protein is a novel autoantigen in Behcet's disease

• 发表时间: 2007
• 作者: 奥貫 陽子