Mechanisms of transcriptional initiation and regulation mediated by general transcription factor TFIID

通用转录因子TFIID介导的转录起始和调控机制

• 批准号: 20370071
• 负责人: KOKUBO Tetsuro
• 金额: $ 12.98万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20370071/
• 关键词: 転写調節; 転写因子; 基本転写因子; 転写開始; 出芽酵母; TFIID; TAF; TBP

In eukaryotes, protein-coding genes are transcribed by RNA polymerase II (pol II) together with general transcription factors (GTFs). TFIID, the largest GTF composed of TATA element-binding protein (TBP) and 14 TBP-associated factors (TAFs), plays a critical role in transcription from TATA-less promoters. In metazoans, several core promoter elements other than the TATA element are thought to be recognition sites for TFIID. However, it is unclear whether functionally homologous elements also exist in TATA-less promoters in Saccharomyces cerevisiae. Here, we identify the cis-elements required to support normal levels of transcription and accurate initiation from sites within the TATA-less and TFIID-dependent RPS5 core promoter. Systematic mutational analyses show that multiple AT-rich sequences are required for these activities and appear to function as recognition sites for TFIID. A single copy of these sequences can support accurate initiation from the endogenous promoter, indicating that they carry highly redundant functions. These results show a novel architecture of yeast TATA-less promoters and support a model in which pol II scans DNA downstream from a recruited site, while searching for appropriate initiation site(s).

在真核生物中，蛋白质编码基因由RNA聚合酶II（pol II）与一般转录因子（GTF）一起转录。TFIID是由TATA元件结合蛋白（TBP）和14个TBP相关因子（TAF）组成的最大的GTF，在TATA缺失启动子的转录中起着关键作用。在后生动物中，除了TATA元件之外的几个核心启动子元件被认为是TFIID的识别位点。然而，目前还不清楚是否功能同源元件也存在于酿酒酵母中的TATA-少启动子。在这里，我们确定的顺式元件，以支持正常水平的转录和准确的启动内的TATA和TFIID依赖性RPS 5核心启动子的网站。系统突变分析表明，这些活动需要多个富含AT的序列，并且似乎充当TFIID的识别位点。这些序列的单个拷贝可以支持从内源启动子的准确起始，表明它们携带高度冗余的功能。这些结果显示了酵母TATA-少启动子的新结构，并支持pol II从募集位点扫描DNA下游，同时寻找合适的起始位点的模型。

项目成果

Hmol directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.

Hmol 通过掩盖无核小体区域，将预起始复合物组装引导至其靶基因启动子上的适当位点。

• 发表时间: 2011
• 期刊: Nucleic Acids Research
• 影响因子: 14.9
• 作者: Kasahara K;Ohyama Y;Kokubo T.
• 通讯作者: Kokubo T.

Structure–function correlation of micro1 for micromere specification in sea urchin embryos

海胆胚胎微粒规范的 micro1 的结构-功能相关性

• 发表时间: 2009
• 期刊: Mechanisms of Development
• 影响因子: 2.6
• 作者: A. Yamazaki;Sewon Ki;T. Kokubo;M. Yamaguchi
• 通讯作者: M. Yamaguchi

TFIIDサブユニットのゲノムワイドなプロモーター結合解析

TFIID 亚基的全基因组启动子结合分析

• 发表时间: 2008
• 作者: 大槻和重;ら
• 通讯作者: ら

Deletion of the N-terminal domain of TAF1 (taf1-ΔTAND) exhibits synthetic lethality with mutations of the RAM signaling network in budding yeast.

TAF1 N 端结构域 (taf1-ΔTAND) 的缺失在芽殖酵母中通过 RAM 信号网络的突变表现出合成致死性。

• 发表时间: 2009
• 作者: Y. Ohyama;K.Kasahara;T.Kokubo
• 通讯作者: T.Kokubo

Hmo1 directs preinitiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region.

Hmo1 通过掩盖无核小体区域，将起始前复合物组装引导至其靶基因启动子上的适当位点。

• 发表时间: 2011
• 期刊: Nucleic Acids Res. vol.39, No.10
• 作者: K. Kasahara;Y. Ohyama;T. Kokubo
• 通讯作者: T. Kokubo