Roles of cytokines and TLRs in lymphocyte activation and differentiation

细胞因子和 TLR 在淋巴细胞活化和分化中的作用

• 批准号: 20390141
• 负责人: TAKATSU Kiyoshi
• 金额: $ 12.73万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390141/
• 关键词: リンパ球; IL-5; TLR; 抗原交叉提示; 免疫学; 自然免疫; IFN-γ; Iigp1

To investigate IL-5-producing cells in the peripheral tissues, we succeeded to generate IL-5/GFP knock-in mice. IL-5/GFP^+ cells were distributed mainly in the lung and intestinal tissues and they were CD3^- c-kit^- cells but not CD3^+ T cells. We also analyzed RP105 signals in B cell activation and found that RP105 was required for TLR4-dependent plasma cell differentiation and IgM production. Finally, we demonstrated that an interferon-inducible gene Iigp1 had a crucial role in Th1-dependent antigen cross-presentation.

为了研究外周组织中产生IL-5的细胞，我们成功地建立了IL-5/GFP敲入小鼠。IL-5/GFP^+细胞主要分布于肺和肠道组织，为CD3^-c-kit^-T细胞，而不是CD3^+T细胞。我们还分析了B细胞激活过程中的RP105信号，发现RP105是TLR4依赖的浆细胞分化和IgM产生所必需的。最后，我们证明了干扰素诱导基因Iigp1在Th1依赖的抗原交叉提呈中起着关键作用。

项目成果

An adaptor protein Lnk/Sh2B3, negative regulator of lymphohematopoiesis, contributes to the maintenance of gut-associated lymphoid tissues.

接头蛋白 Lnk/Sh2B3 是淋巴造血的负调节因子，有助于维持肠道相关淋巴组织。

• 发表时间: 2009
• 作者: Katayama;H.
• 通讯作者: H.

Growth and maturation of megakaryocytes is regulated by Lnk/Sh2b3 adaptor protein through crosstalk between cytokines-and integrin-mediated signals.

Lnk/Sh2b3 接头蛋白通过细胞因子和整合素介导的信号之间的串扰来调节巨核细胞的生长和成熟。

• 发表时间: 2008
• 期刊: Experimental Hematology Vol.36
• 作者: Takizawa H.;Takatsu K.
• 通讯作者: Takatsu K.

Identification of soluble MD-1 and its role in autoimmune diseases.

可溶性 MD-1 的鉴定及其在自身免疫性疾病中的作用。

• 发表时间: 2008
• 作者: Sasaki;S.
• 通讯作者: S.

The maintenance of gut-associated lymphoid tissues regulated by Lnk/Sh2B3 adaptor, a shared risk factor gene associated with type 1 diabetes and celiac disease

Lnk/Sh2B3 接头调节肠道相关淋巴组织的维持，Lnk/Sh2B3 接头是与 1 型糖尿病和乳糜泻相关的共有危险因素基因

• 发表时间: 2010
• 作者: 片山緩子、井関將典;他
• 通讯作者: 他

Local microbleeding facilitates IL-6- and IL-17-dependent arthritis in the absence of tissue antigen recognition by activated T cells.

• DOI: 10.1084/jem.20100900
• 发表时间: 2011-01-17
• 期刊: The Journal of experimental medicine
• 作者: Murakami M;Okuyama Y;Ogura H;Asano S;Arima Y;Tsuruoka M;Harada M;Kanamoto M;Sawa Y;Iwakura Y;Takatsu K;Kamimura D;Hirano T
• 通讯作者: Hirano T