Construction of the system for hematopoietic cell production from human ES cells and the analysis of its molecular basis toward the development of ES cell therapies

本研究分析了TAL1/SCL基因转导对造血细胞分化的作用机制，以期开发利用人类胚胎干细胞的新疗法。我们通过慢病毒基因转移建立了组成性表达Tal1/scl基因的ES细胞系，并证明该细胞系能产生各种类型的造血祖细胞集落。并利用高效的人胎肝慢病毒cDNA文库，筛选出与Tal1/scl基因协同的促进造血功能的候选基因。我们已经克隆了29个候选基因，现在正在精确地研究它们在造血中的作用。这些结果将为今后人类ES细胞的临床应用提供重要信息。

In this study, we analyzed the mechanism for hematopoietic cell differentiation by Tal1/Scl gene-transduction for the purpose of developing new therapies using human embryonic stem(ES) cells. We established the ES cell line expressing Tal1/Scl gene constitutively by lentiviral gene transfer and demonstrated that these cell lines produced various types of hematopoietic progenitor cell colonies. And we screened the candidate genes which promote the hematopoiesis as the collaboration with Tal1/Scl gene using high-performance human fetal liver-derived lentiviral cDNA library. We have cloned twenty-nine candidate genes and are now studying their roles in hematopoiesis precisely. These results would give us important information toward the clinical application of human ES cells in future.