Identification of a novel chimera gene not involving PAX family gene in rhabdomyosarcoma cell lines

横纹肌肉瘤细胞系中不涉及PAX家族基因的新型嵌合体基因的鉴定

• 批准号: 20590365
• 负责人: KAKAZU Naoki
• 金额: $ 3.08万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590365/
• 关键词: 横紋筋肉腫; 染色体転座; 病型特異的転座; キメラ遺伝子

Rhabdomyosarcoma(RMS) is characterized by the specific chromosomal translocations involving 2q35 and 1p36 bands, which generate the PAX3-and PAX7-related chimera genes, respectively. We analyzed RMS cell lines by spectral karyotyping(SKY) and detected many chromosomal translocations, in which the PAX3 and PAX7 genes were not involved. We analyzed their breakpoint regions by FISH but could not identify novel chimera genes, which suggests that the translocations were secondarily caused by chromosomal instability due to the RMS development.

横纹肌肉瘤（RMS）的特点是涉及2q35和1p36条带的特定染色体易位，分别产生PAX3和PAX7相关嵌合基因。我们通过光谱核型分析（SKY）分析了RMS细胞系，检测到许多染色体易位，其中不涉及PAX3和PAX7基因。我们通过 FISH 分析了它们的断点区域，但无法识别新的嵌合体基因，这表明易位其次是由 RMS 发育引起的染色体不稳定引起的。

项目成果

Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors.

通过位点特异性重组酶和基于荧光蛋白的载体进行染色体操作。

• DOI: 10.1371/journal.pone.0009846
• 发表时间: 2010-03-24
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Uemura M;Niwa Y;Kakazu N;Adachi N;Kinoshita K
• 通讯作者: Kinoshita K

Restoration of p53 Pathway by Nutlin-3 Induces Cell Cycle Arrest and Apoptosis in Human Rhabdomyosarcoma Cells

• DOI: 10.1158/1078-0432.ccr-08-2955
• 发表时间: 2009-06-15
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Miyachi, Mitsuru;Kakazu, Naoki;Hosoi, Hajime
• 通讯作者: Hosoi, Hajime

The chromosomal constitution of postmitotic neurons, assessed by neuronal nuclear transfer into oocytes and in ES cell lines derived from them

有丝分裂后神经元的染色体构成，通过神经元核转移到卵母细胞及其衍生的 ES 细胞系中进行评估

• DOI: 10.1159/000230004
• 发表时间: 2009
• 期刊: Cytogenet Genome Res
• 影响因子: 1.7
• 作者: T. Osada;N. Kakazu;M. Watanabe;H. Yamane;T. Yagi
• 通讯作者: T. Yagi

A novel PAX3 rearrangement in embryonal rhabdomyosarcoma

胚胎横纹肌肉瘤中的新型 PAX3 重排

• 发表时间: 2009
• 期刊: Cancer Genet Cytogenet 189(2)
• 作者: Hosoi H;Kakazu N;Konishi E;(他18名)
• 通讯作者: (他18名)