Identification and analysis of nucleocytoplasmic transport pathway using our novel assay system

使用我们的新型检测系统鉴定和分析核细胞质转运途径

• 批准号: 20770166
• 负责人: KOSE Shingo
• 金额: $ 2.75万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770166/
• 关键词: 核-細胞質間分子輸送; 熱ショック応答; Importin; 核内移行; 分子シャペロン; importin; 熱ショック

Previous studies suggest that the conventional nucleocytoplasmic transport, which is mediated by members of Importin β family, is down-regulated under cellular stress conditions, whereas molecular chaperones Hsc70/Hsp70 is well known to accumulate rapidly into the nucleus in response to heat shock. However, this mechanisms translocating into the nucleus remain elusive. In this study, we purified biochemically a cytosolic protein that is essential for nuclear import of Hsc70/Hsp70. Our data suggest that this molecule is a novel nuclear import receptor for Hsc70/Hsp70.

以往的研究表明，由Importin β家族成员介导的常规核质转运在细胞应激条件下下调，而分子伴侣Hsc 70/Hsp 70在热休克反应中迅速积聚到细胞核中。然而，这种转移到细胞核中的机制仍然难以捉摸。在这项研究中，我们纯化的细胞溶质蛋白，是必不可少的核进口的Hsc 70/Hsp 70。我们的数据表明，该分子是一种新的核输入受体Hsc 70/Hsp 70。

项目成果

入門ケミカルバイオロジー

化学生物学导论

• 发表时间: 2008
• 作者: Morishima;S.;長田裕之
• 通讯作者: 長田裕之

Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain.

• DOI: 10.1093/hmg/ddn239
• 发表时间: 2008-11-15
• 期刊: HUMAN MOLECULAR GENETICS
• 影响因子: 3.5
• 作者: Hatayama, Minoru;Tomizawa, Tadashi;Sakai-Kato, Kumiko;Bouvagnet, Patrice;Kose, Shingo;Imamoto, Naoko;Yokoyama, Shigeyuki;Utsunomiya-Tate, Naoko;Mikoshiba, Katsuhiko;Kigawa, Takanori;Aruga, Jun
• 通讯作者: Aruga, Jun

A live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells.

一种实时成像系统，用于可视化哺乳动物细胞间期核孔复合体 (NPC) 的形成。

• 发表时间: 2010
• 期刊: Genes Cells (in press)
• 作者: Iino;H.;Maeshima;K.;Nakatomi;R.;Kose;S.;Hashikawa;T.;Tachibana;T.;Imamoto;N.
• 通讯作者: N.

熱ショック時における分子シャペロンHsc70の核内移行を促進するタンパク質の同定Identification of a protein facilitating nuclear import of molecular chaperone Hsc70 under heat shock

热激下促进分子伴侣 Hsc70 核输入的蛋白质的鉴定

• 发表时间: 2009
• 作者: 小瀬真吾;今本尚子
• 通讯作者: 今本尚子

Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport

• DOI: 10.1038/nsmb.1656
• 发表时间: 2009-10-01
• 期刊: NATURE STRUCTURAL & MOLECULAR BIOLOGY
• 影响因子: 16.8
• 作者: Kosako, Hidetaka;Yamaguchi, Nozomi;Hattori, Seisuke
• 通讯作者: Hattori, Seisuke