Elucidation of oxidative stress damage repair mechanism of budding yeast based on comprehensive phenotype analysis.

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Based on comprehensive phenotype analysis using the complete collection of mutant strains deleted for each of all non-essential genes of budding yeast, I revealed that genes involved in vacuolar acidification, DNA-repair and mitochondrial function were required for the growth after exposure to oxidative stress. The function of V-ATPase, which is responsible for vacuolar acidification, was found to be indispensable for tolerance to multiple stresses. Analysis of deletion mutants that exhibited tolerance to oxidative stress suggested the possibility that deletions of a part of genes involved in vacuolar function and peroxisome function confer tolerance to oxidative stress.