Elucidation of repair mechanism for oxidative damage in budding yeast based on genome-wide phenotype analyses
基于全基因组表型分析阐明芽殖酵母氧化损伤的修复机制
基本信息
- 批准号:23510250
- 负责人:
- 金额:$ 3.33万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2013
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To identify gene functions involved in repair mechanism for oxidative damage in budding yeast Saccharomyces cerevisiae,we conducted genome-wide phenotype analyses using the complete collection of mutant strains deleted for each of all non-essential genes of budding yeast and the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library. The results suggest that genes related to RNA polymerases and the Ubiquitin-Proteasome system, as well as vacuolar acidification that were identified to be critical for tolerance to oxidative stress in our previous study, play important roles in tolerance to oxidative stress. We constructed a systematic library for comprehensive overexpression screens in S. cerevisiae using the systematic collection of yeast genome in a high-copy vector. Using this overexpression library, we screened strains that showed resistance or sensitivity to oxidative stress.
为了确定芽期酵母氧化损伤修复机制中涉及的基因功能,我们利用芽期酵母所有非必需基因的完整缺失突变株和通过mRNA扰动(DAMP)减少的丰度酵母文库进行了全基因组表型分析。这些结果表明,与RNA聚合酶和泛素-蛋白酶体系统相关的基因,以及在我们先前的研究中被确定为对氧化应激耐受至关重要的空泡酸化,在对氧化应激的耐受中起着重要的作用。我们利用高拷贝载体中酵母基因组的系统收集,构建了一个在酿酒酵母中进行全面过表达筛选的系统文库。使用这个过表达文库,我们筛选出对氧化应激表现出抗性或敏感性的菌株。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
パンづくりを支える微生物機能-酵母と乳酸菌を中心にして-
支持面包制作的微生物功能 - 关注酵母和乳酸菌 -
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:島純;小松崎典子;吉田綾子;安藤聡;中村敏英
- 通讯作者:中村敏英
Improvement of ethanol tolerance by overexpression of genes involved in yeast vacuolar ATPases (V-ATPase) function
通过过度表达参与酵母液泡 ATP 酶(V-ATP 酶)功能的基因来改善乙醇耐受性
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:長谷川園子;安藤聡;小川順;高木博史;島純
- 通讯作者:島純
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ANDO Akira其他文献
Simultaneous Measurements of Local Axial and Radial Momentum Fluxes near a Radial Wall of a Helicon Source
同时测量螺旋源径向壁附近的局部轴向和径向动量通量
- DOI:
10.1585/pfr.14.1301143 - 发表时间:
2019 - 期刊:
- 影响因子:0.8
- 作者:
SUGAWARA Takeharu;TAKAHASHI Kazunori;ANDO Akira - 通讯作者:
ANDO Akira
ANDO Akira的其他文献
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{{ truncateString('ANDO Akira', 18)}}的其他基金
Development of a high-intensity optical cavity and a photo-neutralizer for high energy negative ion beams
开发用于高能负离子束的高强度光学腔和光中和器
- 批准号:
18H01197 - 财政年份:2018
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
New concept of electric propulsion using a liquid metal sputtering
使用液态金属溅射的电力推进新概念
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18K18907 - 财政年份:2018
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Study on Work-Life Design focusing on Quality of Work
以工作质量为中心的工作生活设计研究
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16K12669 - 财政年份:2016
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of the new type ion engine using a reflected-type negative ion source
使用反射式负离子源的新型离子发动机的开发
- 批准号:
24654187 - 财政年份:2012
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Performance enhancement of actively-controllable hydrogen/deuterium negative ion source
主动可控氢/氘负离子源性能增强
- 批准号:
23360408 - 财政年份:2011
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Therapeutic approaches targeting colonic myofibroblasts-derived IL-24 in inflammatory bowel disease.
针对炎症性肠病中结肠肌成纤维细胞衍生的 IL-24 的治疗方法。
- 批准号:
21590809 - 财政年份:2009
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Emission of the greenhouse gas to the outside of the earth using plasma acceleration
利用等离子体加速将温室气体排放到地球外部
- 批准号:
21654083 - 财政年份:2009
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Elucidation of oxidative stress damage repair mechanism of budding yeast based on comprehensive phenotype analysis.
基于综合表型分析阐明芽殖酵母氧化应激损伤修复机制
- 批准号:
20780065 - 财政年份:2008
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of heating and acceleration technology in a fast-flowing plasma and application to an advanced space plasma thruster
快速流动等离子体加热和加速技术的开发及其在先进空间等离子体推进器中的应用
- 批准号:
18340183 - 财政年份:2006
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Ion heating in a fast-flowing plasma for applying an advanced space propulsion
快速流动等离子体中的离子加热用于应用先进的空间推进
- 批准号:
15340196 - 财政年份:2003
- 资助金额:
$ 3.33万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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