Elucidation of repair mechanism for oxidative damage in budding yeast based on genome-wide phenotype analyses

为了确定出芽酵母氧化损伤修复机制中涉及的基因功能，我们利用出芽酵母所有非必需基因缺失的突变菌株的完整集合和mRNA微扰（DAmP）酵母文库进行了全基因组表型分析。结果表明，RNA聚合酶和泛素-蛋白酶体系统相关基因以及空泡酸化在我们之前的研究中被确定为对氧化应激耐受至关重要的基因在氧化应激耐受中发挥重要作用。利用酵母基因组在高拷贝载体上的系统收集，构建了酿酒酵母过表达筛选系统文库。利用这个过表达文库，我们筛选了对氧化应激具有抗性或敏感性的菌株。

To identify gene functions involved in repair mechanism for oxidative damage in budding yeast Saccharomyces cerevisiae,we conducted genome-wide phenotype analyses using the complete collection of mutant strains deleted for each of all non-essential genes of budding yeast and the Decreased Abundance by mRNA Perturbation (DAmP) Yeast Library. The results suggest that genes related to RNA polymerases and the Ubiquitin-Proteasome system, as well as vacuolar acidification that were identified to be critical for tolerance to oxidative stress in our previous study, play important roles in tolerance to oxidative stress. We constructed a systematic library for comprehensive overexpression screens in S. cerevisiae using the systematic collection of yeast genome in a high-copy vector. Using this overexpression library, we screened strains that showed resistance or sensitivity to oxidative stress.