Analysis of epithelial integrity system in tumor invasion and metastasis of oral cancer

口腔癌肿瘤侵袭转移中上皮完整性系统分析

• 批准号: 21592526
• 负责人: HIGASHIKAWA Koichiro
• 金额: $ 2.83万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592526/
• 关键词: 口腔癌; 上皮間葉移行; 癌の浸潤・転移; p^63; 上皮整合性; 上皮・間葉移行; p63

Epithelial-Mesencymal Transition(EMT) is involved in tumor invasion and metastasis of oral squamous cell carcinomas(SCCs). Snail, a transcription factor and EMT inducer, directs transcription of its target genes for the acquisition of EMT in SCC cells. We focused a process of loss of epithelial integrity among SCC cells during the EMT. For the investigation of that, we generated SCC cell pools of forced Snail expression by recombinant virus infection system. Almost the cells displayed constant expression of exogenous Snail, however, a small population of the cells underwent EMT. The cells on culture dish were given the migration area and were escalated into EMT. Immnocytochemistory and immnoblot analyses showed that loss of epithelial integrity and cell-cell contact were the last step of EMT process and the process was accelerated by distinct humoral factors.

上皮-间质转化（EMT）参与口腔鳞状细胞癌（SCC）的侵袭和转移。Snail是一种转录因子和EMT诱导因子，它指导其靶基因的转录以在SCC细胞中获得EMT。我们关注EMT过程中SCC细胞上皮完整性丧失的过程。为了研究这一点，我们通过重组病毒感染系统产生了强制Snail表达的SCC细胞池。大多数细胞显示外源Snail的恒定表达，然而，一小部分细胞经历EMT。将培养皿上的细胞给予迁移区并逐步升级为EMT。免疫细胞化学和免疫印迹分析表明，上皮完整性的丧失和细胞间的接触是EMT过程的最后一步，不同的体液因素加速了EMT过程。

项目成果

Functional analysis of Snail-mediated downregulation of deltaNp63alpha in squamous cell carcinoma cells

Snail 介导的鳞状细胞癌细胞中 deltaNp63alpha 下调的功能分析

• 发表时间: 2009
• 作者: 東川晃一郎;他
• 通讯作者: 他

Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas

• DOI: 10.3892/ijo_00000286
• 发表时间: 2009-06-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Shigeishi, Hideo;Fujimoto, Shinichi;Kamata, Nobuyuki
• 通讯作者: Kamata, Nobuyuki

Lymphangiogenesis and metastasis : Role of tumor-inducedlymphatic vessel activation in extramammary Paget' s disease

淋巴管生成和转移:肿瘤诱导的淋巴管激活在乳房外佩吉特病中的作用

• 发表时间: 2009
• 期刊: Am JPathol
• 作者: Hirakawa S;Detmar M;Kerjaschki D;Nagamatsu S;Matsuo K;Tanemura A;Kamata N;Higashikawa K;Okazaki H;Kameda K;Nishida-Fukuda H;Mori H;Hanakawa Y;Sayama K;Shirakata Y;Tohyama M;Tokumaru S;Katayama I;Hashimoto K
• 通讯作者: Hashimoto K

Snailによる転写調節とEMTの誘導

Snail 的转录调控和 EMT 诱导

• 发表时间: 2009
• 作者: 鎌田伸之;東川晃一郎;飛梅圭
• 通讯作者: 飛梅圭

A Snail target gene product Cyr61 regulates the invasiveness of squamous cellcarcinoma.

Snail 靶基因产物 Cyr61 调节鳞状细胞癌的侵袭性。

• 发表时间: 2010
• 作者: Furukawa R;Yamada Y;Matsushima Y;Goto Y-I;Harashima H.;田中扶美
• 通讯作者: 田中扶美