Single-cell microscopy with stimulus and response device to analyze relaxation mechanism of ES cells fluctuation

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The mechanism that provides stability during embryonic development remains to be uncovered. To do this, we made a system to observe a single-cell reaction to culture medium stimulation. The system consists of four parts : ES cells, which are a good model for early development, a culture medium without any unknown factors, a specially coated culture dish that enabled us to dissociate cells without aggregation, and time-lapse microscopy. This system enabled us to monitor life-and-death, proliferation, and differentiation of a single ES cell. Single-cell level observation of stimulation response of ES cells will deepen our understanding of developmental stability.