Single-cell microscopy with stimulus and response device to analyze relaxation mechanism of ES cells fluctuation

带有刺激和反应装置的单细胞显微镜分析ES细胞波动的弛豫机制

• 批准号: 21602001
• 负责人: OHNUMA Kiyoshi
• 金额: $ 3.16万
• 依托单位: Nagaoka University of Technology (2010-2011)The University of Tokyo (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21602001/
• 关键词: 1細胞培養; ES細胞; ゆらぎ; 微細加工; 初期発生; 一細胞培養; 揺らぎ; 無血清培養; E-カドヘリン

The mechanism that provides stability during embryonic development remains to be uncovered. To do this, we made a system to observe a single-cell reaction to culture medium stimulation. The system consists of four parts : ES cells, which are a good model for early development, a culture medium without any unknown factors, a specially coated culture dish that enabled us to dissociate cells without aggregation, and time-lapse microscopy. This system enabled us to monitor life-and-death, proliferation, and differentiation of a single ES cell. Single-cell level observation of stimulation response of ES cells will deepen our understanding of developmental stability.

在胚胎发育过程中提供稳定性的机制仍有待发现。为此，我们制作了一个系统来观察单细胞对培养基刺激的反应。该系统由四部分组成：胚胎干细胞，这是一个很好的早期发育模型，没有任何未知因素的培养基，一个特殊涂层的培养皿，使我们能够分离细胞而不聚集，以及延时显微镜。该系统使我们能够监测单个胚胎干细胞的生死、增殖和分化。单细胞水平观察胚胎干细胞的刺激反应将加深我们对发育稳定性的理解。

项目成果

Generation of Human Induced Pluripotent Stem Cells from Adult Dermal Fibroblast under Feeder-and Serum-free Defined Conditions

在无饲养层和无血清的限定条件下从成体真皮成纤维细胞生成人类诱导多能干细胞

• 发表时间: 2011
• 作者: Hayashi Y.;Chan T.;Warashina M.;Fukuda M.;Ariizumi T.;Okabayashi K.;Takayama N.;Otsu M.;Eto K.;Furue MK.;Michiue T.;Ohnuma K.;Nakauchi H.;Asashima M
• 通讯作者: Asashima M

Inhibitory Smad proteins promote the differentiation of mouse embryonic stem cells into ependymal-like ciliated cells.

• DOI: 10.1016/j.bbrc.2010.08.099
• 发表时间: 2010-10
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Y. Nishimura;A. Kurisaki;Mio Nakanishi;K. Ohnuma;Naoto Ninomiya;S. Komazaki;S. Ishiura;M. Asashima

Mechanosensing Biology, 1st Edition., Noda, M.(Ed.)

机械传感生物学，第一版，Noda, M.（主编）

• 发表时间: 2011
• 作者: M. Asashima;T. Michiue;K. Ohnuma;Y. Nakajima;Y. Ito
• 通讯作者: Y. Ito

Directional migration of neuronal PC12 cells in a ratchet wheel shaped microchamber

神经元 PC12 细胞在棘轮形微室中的定向迁移

• 发表时间: 2009
• 期刊: Journal of Bioscience and Bioengineering 108(1)
• 作者: Ohnuma;K.;Toyota;T.;Ariizumi;T.;Sugawara;T.;Asashima;M.
• 通讯作者: M.

Time-lapse imaging of mES cells monolayer-cultured for the detection of fluctuations between differentiated and undifferentiated states

单层培养的 mES 细胞延时成像，用于检测分化和未分化状态之间的波动

• 发表时间: 2011
• 作者: A Maruyama;Y Wakamoto;K Oonuma
• 通讯作者: K Oonuma