NMR structural analysis for the whole structure of human general transcription factor, TFIIE.

本研究是对人类TFIIE蛋白质编码基因转录起始所必需的转录因子的整体结构进行NMR结构分析。人TFIIE是异二聚体，由α和β亚基组成。虽然除了高分子量（~ 80 kDa）之外的聚集趋势使得结构分析困难，但是充分利用最新的NMR技术能够获得三个功能域的结构信息，识别广泛存在的内在无序区域并揭示动力学。

This study was the NMR structural analysis for the whole structure of human TFIIE, a member of the general transcription factors, needed for the initiation of transcription for protein coding genes. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. Although the tendency to aggregate besides the high molecular weight (~80kDa) made the structural analysis difficult, full use of the latest NMR technologies enabled to gain structural information on three functional domains, identify widely existing intrinsically disordered regions and reveal the dynamics.