NMR structural analysis for the whole structure of human general transcription factor, TFIIE.

本研究对人类TFIIE的全结构进行了核磁共振结构分析，TFIIE是蛋白质编码基因转录启动所需的一种通用转录因子。人TFIIE是一种异源二聚体，由α亚基和β亚基组成。尽管高分子量(~80 kDa)以外的聚集倾向给结构分析带来了困难，但充分利用最新的核磁共振技术能够获得三个功能结构域的结构信息，识别广泛存在的内在无序区域并揭示其动力学。

This study was the NMR structural analysis for the whole structure of human TFIIE, a member of the general transcription factors, needed for the initiation of transcription for protein coding genes. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. Although the tendency to aggregate besides the high molecular weight (~80kDa) made the structural analysis difficult, full use of the latest NMR technologies enabled to gain structural information on three functional domains, identify widely existing intrinsically disordered regions and reveal the dynamics.