NMR study for the whole structure of human general transcription factor TFIIE

一般转录因子是RNA聚合酶II启动蛋白质编码基因转录所必需的。在本研究中，我用核磁共振技术分析了人类转录因子TFIIE的整体结构。人TFIIE是异二聚体，由α和β亚基组成。TFIIE具有大分子量（~ 80 kDa）并且显示聚集的倾向。虽然这些特征不利于通过NMR进行结构分析，但使用最新的NMR技术和高磁场NMR机器能够获得TFIIE的三个功能域的结构信息。我能够确定完整的TFIIE复合物中三个功能域之一的高分辨率三级结构。

General transcription factors are necessary for transcription initiation of protein coding genes by RNA polymerase II. In this study, I structurally analyzed the whole structure of human TFIIE, a member of the general transcription factors by using NMR. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. TFIIE has large molecular weight (~80kDa) and shows the tendency to aggregate. Although these characters are disadvantage for structural analysis by NMR, use of the latest NMR technologies and high magnetic filed NMR machines enabled to gain structural information on three functional domains of TFIIE. I was able to determine a high resolution tertiary structure of one of three functional domains in the intact TFIIE complex.