Molecular breeding of tryptophan-fortified rice via gene targeting

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Gene targeting(GT) via homologous recombination is a powerful transformation technology because it enables to modify the endogenous target gene as expected. In this study, we succeeded in the production of novel tryptophan(Trp)-fortified rice plants by GT-mediated targeted mutagenesis of OASA2 gene that encodes a key enzyme in Trp biosynthesis of rice. In the GT plant, other amino acids except for Trp and the metabolites synthesized from Trp such as serotonin, vitamin B and indole alkaloid accumulated at a higher level also. Thus, we demonstrated that GT-mediated targeted mutagenesis is applicable to metabolic engineering in crops.