Rolle des Zytoskeletts bei der Endothel-Infektion durch S. aureus

细胞骨架在金黄色葡萄球菌内皮感染中的作用

• 批准号: 5389377
• 负责人: Professor Dr. Martin Aepfelbacher
• 金额: --
• 依托单位: Institut für Medizinische Mikrobiologie, Virologie und Hygiene
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5389377?language=en
• 关键词: Rolle; des; Zytoskeletts; bei; der

Staphylococcus aureus, one of most successful bacterial pathogens, can be found within the endothelium in infected animals and invades endothelial cells in culture. The effects of S. aureus on the endothelium are thought to cause symptoms of sepsis and prolonged survival of the bacteria within endothelial cells may underly chronic inflammation. Endothelial cell invasion is mediated by integrins and staphylococcal fibronectin binding proteins but the molecular events governing uptake are unknown. S. aureus can produce exotoxins which are unable to penetrate cell membranes, suggesting that these may be intracellularly produced. On this background we want to investigate the role of the cytoskeleton, both, during S. aureus uptake and as target of extracellularly and intracellularly secreted S. aureus virulence factors. One focus will be on the function of Rho GTP-binding proteins because these are crucial for bacterial uptake, intracellular transport and constitute substrates of an interesting S. aureus toxin, the epidermal cell differentiation inhibitor (EDIN).

金黄色葡萄球菌是最成功的细菌病原体之一，可以在受感染动物的内皮细胞中发现，并在培养物中侵入内皮细胞。金黄色葡萄球菌对内皮的影响被认为会引起脓毒症症状，而内皮细胞内细菌的长期存活可能是慢性炎症的原因。内皮细胞侵袭是由整合素和葡萄球菌纤连蛋白结合蛋白介导的，但控制摄取的分子事件尚不清楚。金黄色葡萄球菌可以产生无法穿透细胞膜的外毒素，表明这些毒素可能是在细胞内产生的。在此背景下，我们想要研究细胞骨架在金黄色葡萄球菌摄取过程中的作用以及作为细胞外和细胞内分泌的金黄色葡萄球菌毒力因子的靶标的作用。其中一个焦点将是 Rho GTP 结合蛋白的功能，因为它们对于细菌摄取、细胞内运输至关重要，并且构成一种有趣的金黄色葡萄球菌毒素（表皮细胞分化抑制剂 (EDIN)）的底物。