PRODUCTION OF NEW NATURAL PRODUCTS BY BIOSYNTHETIC ENGINEERING

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Skeleton of triterpene is formed from oxidosqualene by triterpene synthase. Many triterpene synthases give tetra- or penta-cyclic products, while marneral synthase and achilleol synthase give a mono-cyclic product. In this study, these two enzymes were expressed in the yeast where di-oxidosqualene was accumulated to obtain a new natural triterpene with two separate rings which was produced by cyclization of di-oxidosqualene from both ends. TLC analysis showed that the target compound was produced by the transformed yeast with squalene epoxidase and achilleol synthase genes.