Gene therapy for neuropathic pain applying SNP pace maker ion-channel (HCN channel) transduction to the spinal cord and dorsal root ganglion

点击翻译按钮获取中文摘要

HCN ion channels also know as a pace-maker channel play an important role on regulating spontaneous firing of central nerve systems at various neuropathic pain state. In this study, we investigated about the mechanisms of the HCN ion channel expression change to the nerve system. And we also investigated that the possibility of dominant-negative HCN ion channel as the source for the gene therapy to chronic neuropathic pain. We found that increase of cyclic AMP concentration decreased the glycosylated HCN ion channels on the plasma membrane. Non-glycosylated (SNP) HCN channels could reach to the plasma membrane of xenopus oocyte, but it did not pass the current. These findings suggested that the modulation of HCN channel expression on the spinal cord might be useful as the new treatment of chronic neuropathic pain.