The effect of Del-1 by in vivo gene therapy

Del-1体内基因治疗的效果

• 批准号: 22792019
• 负责人: KITANO Hisataka
• 金额: $ 2.66万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22792019/
• 关键词: 癌; 遺伝子; 蛋白質; バイオテクノロジー

A nude mouse was injected an oral squamous cell carcinoma cell line : SCCKN at the subcutaneously back and made a transplant tumor. The transplant tumor was treated by the gene therapy of pFasL or pFasL/E3D1 gene. The pFasL or pFasL/E3D1 gene was injected every week. The all mice of control group and/or treated of pFasL gene group were dead of 49^<th> days. But, the survival rate of mice with treated by pFasL/E3D1 was 87% at 49^<th> days. It was confirmed that the dosage of pFasL/E3D1 controls the increase of tumor, and there is a survival benefit in a transplant tumor.

在裸鼠背部皮下注射口腔鳞状细胞癌细胞系：SCCKN，制成移植瘤。采用pFasL或pFasL/E3 D1基因治疗移植瘤。每周注射pFasL或pFasL/E3 D1基因。对照组和/或pFasL基因治疗组小鼠在49天内全部死亡<th>。而pFasL/E3 D1组小鼠49天存活率为87%<th>。证实pFasL/E3 D1的剂量控制肿瘤的增加，并且在移植肿瘤中存在存活益处。

项目成果

Targets in Gene Therapy (ISBN978-953-307-540-2)

基因治疗的目标 (ISBN978-953-307-540-2)

• 发表时间: 2011
• 作者: Hisataka Kitano;Atsushi Mamiya;Chiaki Hidai
• 通讯作者: Chiaki Hidai

The Extraceuar Matrix Protein Del1 Induces Apoptosis via its Epidermal Growth Factor Motif.

细胞外基质蛋白 Del1 通过其表皮生长因子基序诱导细胞凋亡。

• 发表时间: 2010
• 期刊: Biochemical and Biophysical Research Communications
• 影响因子: 3.1
• 作者: Hisataka Kitano;Shinichiro Kokubun;Chiaki Hidai.
• 通讯作者: Chiaki Hidai.

A peptide from Dell protein induced apoptosis in vitro

Dell 蛋白的肽在体外诱导细胞凋亡

• 发表时间: 2011
• 作者: Hisataka Kitano;Atsushi Mamiya;Shinichiro Kokubun;Chiaki Hidai
• 通讯作者: Chiaki Hidai