Evaluation of seed quality using transcript level of thyroid hormone receptors in aquaculture of Japanese smelt

本研究从日本胡瓜鱼中分离出3种甲状腺激素受体（TR）cDNA，并建立了3种TR mRNA的定量体系。胚胎TR转录水平与孵化率呈正相关。这些结果表明，卵TR转录水平是一个有用的指标，评价胚胎质量的日本胡瓜鱼。此外，还认为对排卵雌鱼施用甲状腺激素对提高胡瓜鱼卵的孵化率有影响。

In this study, 3 types of thyroid hormone receptor(TR) cDNA were isolated and quantitative system of each of the 3 types mRNA was developed in Japanese smelt. Positive relationship was found between embryo's TR transcript level and hatching rate. These results suggest that egg TR transcript level is a useful indicator to evaluate quality of embryos in Japanese smelt. In addition, it was considered that administration of thyroid hormone to ovulated female affected improvement of hatching rate of eggs in Japanese smelt.