In vitro production of the Camel heavy chain antibody toward development of alternative antibody medicine

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Camels have single-chain antibody consisting of only heavy chains. Comparing with standard H2L2 antibodies, single-chain antibody have many advantageous chemical features toward antibody-medicine development, such as high solubility in water and/or thermo-stability. In this study we employed chromosomal engineering to produce camel heavy chain antibody in cell culture system. We have successfully cloned the camel chromosome containing whole antibody-gene cluster into cultured cells, however, we could not detect any camel heavy chain antibody in vitro, suggesting incompatibility of camel immune system with others in spite of highly sequence similarity.