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Preparation of cell-based electron-generator

细胞电子发生器的制备

基本信息

批准号：
22650107
负责人：
ISHIHARA Kazuhiko
金额：
$ 2.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650107/
关键词：
バイオ起電デバイスリン脂質ポリマー自発形成ゲル電子移動メディエーターバクテリア細胞ベース起電デバイス電子メディエーターポリマーハイドロゲルシュワネラ菌細胞親和性可逆的ゲル化反応バイオマテリアル細胞親和型ポリマー酸化還元反応細胞固定化電子移動反応

项目摘要

The biological and electrochemical properties of redox phospholipid polymer hydrogel containing electron-generating bacterium(Shewanella oneidensis MR-1) were investigated herein to understand a fundamental knowledge of materials for developing a microbial fuel cell. A water-soluble and amphiphilic phospholipid polymer, poly[ 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene](PMBVF), used as one of the components of a hydrogel matrix for encapsulation of bacteria and an electron transfer mediator, was synthesized. The hydrogel was formed spontaneously and encapsulated shewanella three-dimensionally when an aqueous solution of PMBVF suspended with the bacterium and an aqueous solution of poly(vinyl alcohol)(PVA) were gently mixed together. Viability evaluation demonstrated that the shewanella encapsulated in the PMBVF/PVA hydrogel maintained high viability levels even after long-term storage and the shewanella made good metabolism. The amperometric measurement showed that the PMBVF/PVA hydrogel could maintain the electron transfer efficiency even when shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provided a mild environment for long-term bacterial survival but also maintained the electron transfer efficiency from the shewanella to the electrode. We concluded that the hydrogel/bacteria hybrid biomaterials, such as PMBVN/PVA/shewanella, have promising application to fabricate living cell-based or bacterium-based biodevices.

本文研究了含电子产生菌（Shewanella oneidensis MR-1）的氧化还原磷脂聚合物水凝胶的生物学和电化学性质，以了解用于开发微生物燃料电池的材料的基础知识。合成了一种水溶性两亲性磷脂聚合物聚[ 2-甲基丙烯酰氧乙基磷酰胆碱-co-甲基丙烯酸正丁酯-co-对乙烯基苯基硼酸-co-乙烯基二茂铁]（PMBVF），作为包埋细菌和电子传递介质的水凝胶基质的组分之一。水凝胶自发形成，并封装希瓦氏菌三维时，PMBVF的水溶液悬浮与细菌和聚（乙烯醇）（PVA）的水溶液轻轻地混合在一起。活性评价表明，包封在PMBVF/PVA水凝胶中的希瓦氏菌即使在长期储存后仍保持较高的活性水平，并且希瓦氏菌进行良好的代谢。电流法测试结果表明，PMBVF/PVA水凝胶在包封希瓦氏菌的情况下仍能保持电子传递效率。因此，PMBVF/PVA水凝胶不仅为细菌的长期存活提供了温和的环境，而且还保持了从希瓦氏菌到电极的电子转移效率。我们的结论是，水凝胶/细菌杂化生物材料，如PMBVN/PVA/希瓦氏菌，具有良好的应用前景，以制造活细胞为基础的或细菌为基础的生物器件。