Preparation of cell-based electron-generator

本文研究了含有电子产生细菌（希瓦氏菌MR-1）的氧化还原磷脂聚合物水凝胶的生物学和电化学特性，以了解开发微生物燃料电池所需材料的基本知识。合成了一种水溶性两亲性磷脂聚合物聚[2-甲基丙烯酰氧乙基磷酸胆碱-co-正甲基丙烯酸丁酯-co-对乙烯基苯基硼酸-co-乙烯基二茂铁](PMBVF)，作为细菌包封和电子传递介质的水凝胶基质组分之一。将悬浮在希瓦氏菌上的PMBVF水溶液与聚乙烯醇（PVA）水溶液轻轻混合，水凝胶可以自发形成并三维包裹希瓦氏菌。活力评价结果表明，经PMBVF/PVA水凝胶包封后的希瓦氏菌在长期保存后仍保持较高的活力水平，且希瓦氏菌代谢良好。安培测量结果表明，PMBVF/PVA水凝胶在包裹希瓦氏菌时仍能保持电子传递效率。因此，PMBVF/PVA水凝胶不仅为细菌的长期生存提供了温和的环境，而且保持了希瓦氏菌到电极的电子传递效率。我们认为，水凝胶/细菌混合生物材料（如PMBVN/PVA/shewanella）在制备基于活细胞或细菌的生物器件方面具有广阔的应用前景。

The biological and electrochemical properties of redox phospholipid polymer hydrogel containing electron-generating bacterium(Shewanella oneidensis MR-1) were investigated herein to understand a fundamental knowledge of materials for developing a microbial fuel cell. A water-soluble and amphiphilic phospholipid polymer, poly[ 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene](PMBVF), used as one of the components of a hydrogel matrix for encapsulation of bacteria and an electron transfer mediator, was synthesized. The hydrogel was formed spontaneously and encapsulated shewanella three-dimensionally when an aqueous solution of PMBVF suspended with the bacterium and an aqueous solution of poly(vinyl alcohol)(PVA) were gently mixed together. Viability evaluation demonstrated that the shewanella encapsulated in the PMBVF/PVA hydrogel maintained high viability levels even after long-term storage and the shewanella made good metabolism. The amperometric measurement showed that the PMBVF/PVA hydrogel could maintain the electron transfer efficiency even when shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provided a mild environment for long-term bacterial survival but also maintained the electron transfer efficiency from the shewanella to the electrode. We concluded that the hydrogel/bacteria hybrid biomaterials, such as PMBVN/PVA/shewanella, have promising application to fabricate living cell-based or bacterium-based biodevices.