Preparation of cell-based electron-generator

细胞电子发生器的制备

基本信息

  • 批准号:
    22650107
  • 负责人:
  • 金额:
    $ 2.14万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 财政年份:
    2010
  • 资助国家:
    日本
  • 起止时间:
    2010 至 2011
  • 项目状态:
    已结题

项目摘要

The biological and electrochemical properties of redox phospholipid polymer hydrogel containing electron-generating bacterium(Shewanella oneidensis MR-1) were investigated herein to understand a fundamental knowledge of materials for developing a microbial fuel cell. A water-soluble and amphiphilic phospholipid polymer, poly[ 2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-vinylferrocene](PMBVF), used as one of the components of a hydrogel matrix for encapsulation of bacteria and an electron transfer mediator, was synthesized. The hydrogel was formed spontaneously and encapsulated shewanella three-dimensionally when an aqueous solution of PMBVF suspended with the bacterium and an aqueous solution of poly(vinyl alcohol)(PVA) were gently mixed together. Viability evaluation demonstrated that the shewanella encapsulated in the PMBVF/PVA hydrogel maintained high viability levels even after long-term storage and the shewanella made good metabolism. The amperometric measurement showed that the PMBVF/PVA hydrogel could maintain the electron transfer efficiency even when shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provided a mild environment for long-term bacterial survival but also maintained the electron transfer efficiency from the shewanella to the electrode. We concluded that the hydrogel/bacteria hybrid biomaterials, such as PMBVN/PVA/shewanella, have promising application to fabricate living cell-based or bacterium-based biodevices.
本文研究了含电子产生菌(Shewanella oneidensis MR-1)的氧化还原磷脂聚合物水凝胶的生物学和电化学性质,以了解用于开发微生物燃料电池的材料的基础知识。合成了一种水溶性两亲性磷脂聚合物聚[ 2-甲基丙烯酰氧乙基磷酰胆碱-co-甲基丙烯酸正丁酯-co-对乙烯基苯基硼酸-co-乙烯基二茂铁](PMBVF),作为包埋细菌和电子传递介质的水凝胶基质的组分之一。水凝胶自发形成,并封装希瓦氏菌三维时,PMBVF的水溶液悬浮与细菌和聚(乙烯醇)(PVA)的水溶液轻轻地混合在一起。活性评价表明,包封在PMBVF/PVA水凝胶中的希瓦氏菌即使在长期储存后仍保持较高的活性水平,并且希瓦氏菌进行良好的代谢。电流法测试结果表明,PMBVF/PVA水凝胶在包封希瓦氏菌的情况下仍能保持电子传递效率。因此,PMBVF/PVA水凝胶不仅为细菌的长期存活提供了温和的环境,而且还保持了从希瓦氏菌到电极的电子转移效率。我们的结论是,水凝胶/细菌杂化生物材料,如PMBVN/PVA/希瓦氏菌,具有良好的应用前景,以制造活细胞为基础的或细菌为基础的生物器件。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Layer-by-Layer Building up of Redox Phospholipid Polymer Hydrogel Electrode for Biosensor
生物传感器用氧化还原磷脂聚合物水凝胶电极的逐层构建
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Z. Li;T. Konno;M. Takai;K. Ishihara
  • 通讯作者:
    K. Ishihara
Enzyme oxidase-immobilized phospholipid polymer microparticles for bio図2.ゲルの網目構造(A)と固定化されたシュワネラ菌(B)図3. PMBVF/PVA/シュワネラ菌電極の起電特性fuel cell application
生物用酶氧化酶固定化磷脂聚合物微粒 图2. 凝胶网络结构(A)和固定化希瓦氏菌(B) 图3. PMBVF/PVA/希瓦氏菌电极燃料电池应用的生电特性
  • DOI:
  • 发表时间:
    2011
  • 期刊:
  • 影响因子:
    0
  • 作者:
    X. Lin;T. Konno;M. Takai;K. Ishihara
  • 通讯作者:
    K. Ishihara
Oxidase-immobilized polymer microparticles coated with MPC polymer for biofuel cell application
涂有 MPC 聚合物的氧化酶固定聚合物微粒,用于生物燃料电池应用
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    X. Lin;T. Konno;M. Takai;K. Ishihara
  • 通讯作者:
    K. Ishihara
Fabrication of polymeric electron-transfer mediator/enzyme hydrogel multilayer on an Au electrode in a layer-by-layer process.
  • DOI:
    10.1016/j.bios.2012.01.042
  • 发表时间:
    2012-04
  • 期刊:
  • 影响因子:
    12.6
  • 作者:
    Ziyin Li;T. Konno;M. Takai;K. Ishihara
  • 通讯作者:
    Ziyin Li;T. Konno;M. Takai;K. Ishihara
Enzyme oxidase-immobilized phospholipid polymer microparticles for biofuel cell application
用于生物燃料电池应用的酶氧化酶固定化磷脂聚合物微粒
  • DOI:
  • 发表时间:
    2011
  • 期刊:
  • 影响因子:
    0
  • 作者:
    X.Lin;T.Konno;M.Takai;K.Ishihara
  • 通讯作者:
    K.Ishihara
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ISHIHARA Kazuhiko其他文献

Synthesis of Thereto-Responsive Hydrogel Cross-Linked by Gold Nano Collaid
金纳米胶交联响应性水凝胶的合成
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    GOTO Fumi;YUSA Shin-ichi;MORISHIMA Yotaro;ISHIHARA Kazuhiko
  • 通讯作者:
    ISHIHARA Kazuhiko

ISHIHARA Kazuhiko的其他文献

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{{ truncateString('ISHIHARA Kazuhiko', 18)}}的其他基金

Preparation of biocompatible polymer nanoparticles as cell-function analysis biodevices
作为细胞功能分析生物器件的生物相容性聚合物纳米颗粒的制备
  • 批准号:
    21300176
  • 财政年份:
    2009
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on a formulation of housing policy vision by character of area and renewal policy in suburb from view point of shrinking city
收缩城市视角下分区特征住房政策愿景制定及郊区更新政策研究
  • 批准号:
    18600009
  • 财政年份:
    2006
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DETERMINATION OF MECHANISM FOR MOLECULAR ADHESION TO SOFT TISSUES AND MATERALS PREPARATION
软组织分子粘附机制的测定及材料制备
  • 批准号:
    17300144
  • 财政年份:
    2005
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function of gastro-intestinal mucin-derived acidic oligosaccharaides recognized by anti-mucin monoclonal antibodies
抗粘蛋白单克隆抗体识别的胃肠粘蛋白衍生酸性寡糖的功能
  • 批准号:
    16590629
  • 财政年份:
    2004
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Structure Control and Functions of Biocompatible Polymer Alloy as Biomaterials
生物相容性聚合物合金作为生物材料的结构控制和功能
  • 批准号:
    13480288
  • 财政年份:
    2001
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A systematic development of monoclonal antibodies against mucins of digestive tract and their application to pathophysiological studies
抗消化道粘蛋白单克隆抗体的系统开发及其在病理生理学研究中的应用
  • 批准号:
    12670514
  • 财政年份:
    2000
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
PREPARATION OF NOVEL HEMOCOMPATIBLE HOLLOW FIBER MEMBRANE AND DEVICE FOR SAFETY BLOOD PURIFICATION HOME
新型血液相容性中空纤维膜及安全血液净化装置的制备
  • 批准号:
    10558131
  • 财政年份:
    1998
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
PREPARATION OF NOVEL BIOMEDICAL POLYMERS HAUING BIOMEMBRANE-LIKE SURFACE AND RECOGNITION OF THEIR NON THROMBOGENIC
具有类生物膜表面的新型生物医用聚合物的制备及其非血栓形成的识别
  • 批准号:
    09480250
  • 财政年份:
    1997
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Mucus secretion studies using anti-gastric mucin monoclonal antibodies
使用抗胃粘蛋白单克隆抗体进行粘液分泌研究
  • 批准号:
    07670612
  • 财政年份:
    1995
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preparation of Novel Biocompatible polymeric Additives and Modification of Biomedical Materials Using the Additives
新型生物相容性高分子添加剂的制备及其对生物医用材料的改性
  • 批准号:
    07680934
  • 财政年份:
    1995
  • 资助金额:
    $ 2.14万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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