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DETERMINATION OF MECHANISM FOR MOLECULAR ADHESION TO SOFT TISSUES AND MATERALS PREPARATION

软组织分子粘附机制的测定及材料制备

基本信息

批准号：
17300144
负责人：
ISHIHARA Kazuhiko
金额：
$ 9.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300144/
关键词：
Adhesion Biomaterials Medical adhesives Polymer complex Physical treatment 医療接着材分子間相互作用

项目摘要

In this study the polymer structures were determined to adhere the polymer to tissues. The molecular design of the polymers were carried out with attention to the regulation of cell/materials interactions from of the physical chemistry and the biological both sides. The polymers could easily solidify themselves by the phase transition of the polymers and voluntary organization as far the normal temperature and to realize the adhesion prescribed it. It prescribed the molecular structure of this polymers closely and composed it, and the wound produced the soft tissue adhesion materials of the prototype than adhesion performance and examination of the safety.A thought prepared nanoparticles as a probe for recognizing the specific reaction analytical technique that I found so far; and to prescribe the functional group, which could adhere for a cell, the structure of the ligand molecule. At first the functional groups with various hydrophobicity, electric charge, hydrogen bonding connected to use physicochemical interaction. The water-soluble phospholipid polymers, which did immobilization of the specific functional group was used for surface treatment materials and the polymer solution for making an O/W emulsion and manufactured nanoparticles.The functional groups cold introduce with high-density for suitable interaction with cells. The adhesion of cells and tissues could be achieved. To determine the structure and composition bonded this polymer to the cells, which became single-layered and examined the reply of the cell As well as a form, the number of the increases, the intracellular enzyme activity was measured to consider in particular cell activity It prescribed polymer composition, the chemical structure that did not affect a cell than these. On the other hand, a molecule design about the polymers was carries out to reduce adhesive property and performed examination form the viewpoint of prevention of organization adhesion.

在本研究中，确定聚合物结构以将聚合物粘附到组织上。从物理化学和生物学两个方面对细胞/材料相互作用的调控进行了聚合物的分子设计。聚合物在常温下通过聚合物的相转变和自发组织而容易固化，实现其所规定的粘接性，这紧密地规定了这种聚合物的分子结构，并将其组成，并对伤口产生的软组织粘附材料的原型进行了粘附性能和安全性的比较检测。具体的反应分析技术，我发现到目前为止;和规定的功能团，它可以附着在一个细胞，结构的配体分子。首先将各种官能团用疏水性、电荷性、氢键性连接起来，利用物理化学相互作用。将固定了特定功能基团的水溶性磷脂聚合物用于表面处理材料，并将聚合物溶液用于制备O/W乳液和制造纳米颗粒，以高密度引入功能基团以适合与细胞的相互作用。可实现细胞与组织的粘附。为了确定结构和组合物键合这种聚合物的细胞，它成为单层和检查的答复的细胞以及一种形式，数量的增加，细胞内酶的活性进行了测量，以考虑特别是细胞活性它规定的聚合物组合物，化学结构，不影响细胞比这些。另一方面，对聚合物进行了分子设计，以降低粘附性能，并从防止组织粘附的角度进行了检查。