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DETERMINATION OF MECHANISM FOR MOLECULAR ADHESION TO SOFT TISSUES AND MATERALS PREPARATION

软组织分子粘附机制的测定及材料制备

基本信息

批准号：
17300144
负责人：
ISHIHARA Kazuhiko
金额：
$ 9.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300144/
关键词：
Adhesion Biomaterials Medical adhesives Polymer complex Physical treatment 医療接着材分子間相互作用

项目摘要

In this study the polymer structures were determined to adhere the polymer to tissues. The molecular design of the polymers were carried out with attention to the regulation of cell/materials interactions from of the physical chemistry and the biological both sides. The polymers could easily solidify themselves by the phase transition of the polymers and voluntary organization as far the normal temperature and to realize the adhesion prescribed it. It prescribed the molecular structure of this polymers closely and composed it, and the wound produced the soft tissue adhesion materials of the prototype than adhesion performance and examination of the safety.A thought prepared nanoparticles as a probe for recognizing the specific reaction analytical technique that I found so far; and to prescribe the functional group, which could adhere for a cell, the structure of the ligand molecule. At first the functional groups with various hydrophobicity, electric charge, hydrogen bonding connected to use physicochemical interaction. The water-soluble phospholipid polymers, which did immobilization of the specific functional group was used for surface treatment materials and the polymer solution for making an O/W emulsion and manufactured nanoparticles.The functional groups cold introduce with high-density for suitable interaction with cells. The adhesion of cells and tissues could be achieved. To determine the structure and composition bonded this polymer to the cells, which became single-layered and examined the reply of the cell As well as a form, the number of the increases, the intracellular enzyme activity was measured to consider in particular cell activity It prescribed polymer composition, the chemical structure that did not affect a cell than these. On the other hand, a molecule design about the polymers was carries out to reduce adhesive property and performed examination form the viewpoint of prevention of organization adhesion.

在这项研究中，确定了聚合物的结构，以将聚合物附着到组织上。聚合物的分子设计从物理化学和生物两方面考虑了细胞/材料相互作用的调节。聚合物在常温下容易通过聚合物的相变和自组织而固化，并实现所规定的粘附性。它紧密地规定了这种聚合物的分子结构并组成了它，并在伤口处产生了软组织黏附材料的原型，通过对黏附性能和安全性的检查。一种思想制备了纳米颗粒作为探针，用于识别我迄今所发现的特定的反应分析技术；并规定了可以黏附于细胞的官能团、配体分子结构。首先将具有不同疏水性、电荷、氢键的官能团通过物理化学反应连接起来。将固定特定官能团的水溶性磷脂聚合物作为表面处理材料和制备O/W乳状液的聚合物溶液，制成纳米粒子，引入密度高的官能团，与细胞进行适当的相互作用。可以实现细胞与组织的黏附。为了确定这种聚合物结合到细胞上的结构和组成，细胞变成单层，并检查细胞的应答以及形式、增加的数量，测量细胞内酶活性，以特别考虑它规定的聚合物组成的细胞活性，即不影响细胞的化学结构。另一方面，对聚合物进行了降低粘接性能的分子设计，并从防止组织粘连的角度进行了检验。