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Preparation of Novel Biocompatible polymeric Additives and Modification of Biomedical Materials Using the Additives

新型生物相容性高分子添加剂的制备及其对生物医用材料的改性

基本信息

批准号：
07680934
负责人：
ISHIHARA Kazuhiko
金额：
$ 1.41万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

To improve the blood compatibility of a segmented polyurethane (SPU), 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was blended in the SPU.The blended membranes composed of SPU and MPC polymers were prepared by a solvent evaporation method. Surface analysis of the SPU membrane blended with the MPC polymer (SPU/MPC polymer) revealed that the MPC polymer was concentrated at the surface of the SPU membrane which contacted the substrate compared with that which contacted air during the membrane formation period. The size of the domains of the MPC polymer became smaller but the number of the domains increased. The amount of the MPC polymer mexed with SPU affected the dispersion state. The mechanical properties of the SPU membrane, as determined by tensile stress-strain measurements, changed very little even after addition of the MPC polymers. Plasma proteins adsorbed on the SPU/MPC polymer membrane surface after contact with human plasma were detected by gold-colloid labeled immunoassay. Both albumin and fibrinogen were observed on the SPU membrane ; however, the amount of these proteins was reduced on the SPU/MPC polymer membrane. It was concluded that the blood compatibility of the SPU was effectively improved by the blending of the MPC polymer. The addition of MPC polymer in the SPU membrane dramatically reduced cell adhesion. It is concluded that the blending of the MPC polymer in the SPU membrane is an effective method to impart nonthrombogenicity. By using of this SPU/MPC polymer blend, small-diameter vascular prosthesis was prepared. The prosthesis indicated excellent performance compared with normal or original prosthesis, that is, during more than 8 months the SPU/MPC polymer blend prosthesis did not osculate even when the prosthesis was implanted into rabbit vain.

为了提高嵌段聚氨酯(SPU)的血液相容性，在SPU中掺入2-甲基丙烯酰氧基乙基磷酰胆碱(MPC)聚合物，采用溶剂蒸发法制备了SPU与MPC聚合物的共混膜。对与MPC聚合物共混的SPU膜(SPU/MPC聚合物)的表面分析表明，与成膜期间接触空气的表面相比，MPC聚合物集中在与基材接触的SPU膜表面。 MPC聚合物的区域尺寸变小，但区域数量增加。 MPC聚合物与SPU混合的量影响分散状态。通过拉伸应力-应变测量确定的 SPU 膜的机械性能即使在添加 MPC 聚合物后也几乎没有变化。采用胶体金标记免疫法检测与人血浆接触后吸附在SPU/MPC聚合物膜表面的血浆蛋白。 SPU膜上同时观察到白蛋白和纤维蛋白原；然而，SPU/MPC 聚合物膜上这些蛋白质的量减少了。结果表明，MPC聚合物的共混有效提高了SPU的血液相容性。在 SPU 膜中添加 MPC 聚合物可显着降低细胞粘附。结论是，在 SPU 膜中共混 MPC 聚合物是赋予非血栓形成性的有效方法。利用这种SPU/MPC聚合物共混物制备了小直径人工血管。与普通或原始假体相比，该假体表现出优异的性能，即在超过8个月的时间里，即使将假体植入兔子体内，SPU/MPC聚合物共混物假体也不会发生接触。