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Preparation of Novel Biocompatible polymeric Additives and Modification of Biomedical Materials Using the Additives

新型生物相容性高分子添加剂的制备及其对生物医用材料的改性

基本信息

批准号：
07680934
负责人：
ISHIHARA Kazuhiko
金额：
$ 1.41万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

To improve the blood compatibility of a segmented polyurethane (SPU), 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was blended in the SPU.The blended membranes composed of SPU and MPC polymers were prepared by a solvent evaporation method. Surface analysis of the SPU membrane blended with the MPC polymer (SPU/MPC polymer) revealed that the MPC polymer was concentrated at the surface of the SPU membrane which contacted the substrate compared with that which contacted air during the membrane formation period. The size of the domains of the MPC polymer became smaller but the number of the domains increased. The amount of the MPC polymer mexed with SPU affected the dispersion state. The mechanical properties of the SPU membrane, as determined by tensile stress-strain measurements, changed very little even after addition of the MPC polymers. Plasma proteins adsorbed on the SPU/MPC polymer membrane surface after contact with human plasma were detected by gold-colloid labeled immunoassay. Both albumin and fibrinogen were observed on the SPU membrane ; however, the amount of these proteins was reduced on the SPU/MPC polymer membrane. It was concluded that the blood compatibility of the SPU was effectively improved by the blending of the MPC polymer. The addition of MPC polymer in the SPU membrane dramatically reduced cell adhesion. It is concluded that the blending of the MPC polymer in the SPU membrane is an effective method to impart nonthrombogenicity. By using of this SPU/MPC polymer blend, small-diameter vascular prosthesis was prepared. The prosthesis indicated excellent performance compared with normal or original prosthesis, that is, during more than 8 months the SPU/MPC polymer blend prosthesis did not osculate even when the prosthesis was implanted into rabbit vain.

为了提高分段聚氨酯（SPU）的血液相容性，在SPU中掺入了2-甲基丙烯酰氧乙基磷酸胆碱（MPC）聚合物。采用溶剂蒸发法制备了SPU和MPC共混膜。对SPU/MPC聚合物共混膜（SPU/MPC聚合物）的表面分析表明，在膜形成过程中，MPC聚合物集中在SPU膜与基质接触的表面，而不是与空气接触的表面。MPC聚合物的结构域变小了，但结构域的数量增加了。MPC聚合物与SPU的掺量对分散状态有影响。通过拉伸应力应变测量，SPU膜的力学性能即使在加入MPC聚合物后也几乎没有变化。用金胶体标记免疫法检测SPU/MPC聚合物膜与人血浆接触后吸附在其表面的血浆蛋白。SPU膜上可见白蛋白和纤维蛋白原；然而，这些蛋白质的数量在SPU/MPC聚合物膜上减少。结果表明，MPC聚合物的掺入有效地改善了SPU的血液相容性。在SPU膜中加入MPC聚合物可显著降低细胞粘附。结果表明，在SPU膜中共混MPC聚合物是一种具有非血栓形成性的有效方法。利用SPU/MPC聚合物共混物制备了小直径血管假体。与正常假体或原假体相比，SPU/MPC聚合物混合假体在8个月以上的时间内即使植入兔体内也没有发生接触。