权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mucus secretion studies using anti-gastric mucin monoclonal antibodies

使用抗胃粘蛋白单克隆抗体进行粘液分泌研究

基本信息

批准号：
07670612
负责人：
ISHIHARA Kazuhiko
金额：
$ 1.15万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670612/
关键词：
anti-mucin antibody monoclonal antibody gastric mucin mucus secretion carbohydrate antigen 糖鐘抗原

项目摘要

Gastric mucin known as a major component of rat gastric mucus is synthesized in and secreted from several different kind of cells present at the mucosal epithelium. It is generally accepted that the regulation mechanism on the secretion and synthesis of mucin in a kind of cells present at the specific region and layr of gastric mucosa differ from the others. It is important to recognaize and estimate the specific mucin of gastric mucosa for understanding the role of mucus in the physiology and pathology of gastric mucosa. In this study we developed several monoclonal antibodies reacting specific mucin molecules and applied these antibodies to establish some methods to study the metabolism of gastric mucins.1) RGM11,21 and 24, are the monoclonal antibodies specifically react with the mucin present at the surface epithelial cells of rat gastric mucosa. It has been demonstrated that RGM11 and 21 recognize the specific oligosaccharide structure of mucin molecule. RGM24 reacts intact mucin molecules but the reactin disappears by the removal of saccharide residue from the mucin molecules.2) We developed 14 novel monoclonal antibodies (MAbs) using purified human colonic mucin as the immunogens (designated as HCM series MAbs). Among these MAbs, 8 MAbs are proved to recognize sialic acid-containing oligosaccaride structure of mucin molecule. In addition, some of these MAbs binding to sialic acid containing sugar chains are specifically react with the intestinal metaplastic mucosa in human stomach.3) We developed a sandwich ELISA mehtod using RGM11 and HIK1083 MAbs to recognize and quantify the surface mucous cell- and gland mucous cell-derived mucin, respectively, included in an extract of single mucosal specimen of rat stomach.

胃粘蛋白是大鼠胃粘液的主要成分，由粘膜上皮中存在的几种不同类型的细胞合成并分泌。人们普遍认为，存在于胃粘膜特定区域和层的一类细胞对粘蛋白分泌和合成的调节机制不同于其他细胞。识别和估计胃粘膜的特异性粘蛋白对于了解粘液在胃粘膜生理和病理中的作用具有重要意义。在本研究中，我们开发了几种与特定粘蛋白分子反应的单克隆抗体，并应用这些抗体建立了一些研究胃粘蛋白代谢的方法。1) RGM11、21和24是与大鼠胃粘膜表面上皮细胞上存在的粘蛋白特异性反应的单克隆抗体。已证明RGM11和21识别粘蛋白分子的特定寡糖结构。 RGM24与完整的粘蛋白分子发生反应，但反应素通过从粘蛋白分子中去除糖残基而消失。2)我们使用纯化的人结肠粘蛋白作为免疫原开发了14种新型单克隆抗体(MAb)(指定为HCM系列MAb)。在这些单克隆抗体中，有8种单克隆抗体被证明能够识别粘蛋白分子中含有唾液酸的寡糖结构。此外，其中一些与含有糖链的唾液酸结合的单克隆抗体与人胃中的肠化生粘膜发生特异性反应。 3）我们使用 RGM11 和 HIK1083 单克隆抗体开发了一种夹心 ELISA 方法，分别识别和定量大鼠胃单个粘膜样本提取物中包含的表面粘液细胞和腺体粘液细胞衍生的粘蛋白。