Reaction Control of Nanoreactor by Utilizing DNA Hybridization

我们利用dna表面活性剂研究了dna导向的囊泡聚集。使用DNA表面活性剂将单链DNA寡核苷酸拴在囊泡上，拴在一起的囊泡与其他带有互补链的囊泡组装在一起。根据DNA杂交的特点，盐浓度和温度对囊泡聚集有强烈的影响。可以水解本研究中使用的双链DNA的限制性内切酶解离了囊泡聚集体。利用荧光标记囊泡的探索表明，dna定向囊泡聚集通过dna双工形成以序列特异性的方式发生。有趣的是，使用短dna表面活性剂的dna定向聚集诱导囊泡融合产生巨囊泡，导致巨囊泡中的酶促反应。

We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.