Reaction Control of Nanoreactor by Utilizing DNA Hybridization

我们使用DNA表面活性剂研究了DNA引导的囊泡聚集。在使用DNA表面活性剂将单链DNA寡核苷酸与囊泡捆绑在一起后，被拴住的囊泡与其他带有互补链的囊泡组装在一起。根据DNA杂交的特点，盐浓度和温度对囊泡聚集有很大的影响。限制性内切酶可以分解双链DNA，使囊泡聚集体解离。使用荧光标记的囊泡的探索表明，DNA引导的囊泡聚集是通过DNA双链形成以序列特异性的方式发生的。有趣的是，使用短DNA表面活性剂的DNA定向聚集诱导了小泡的融合，产生了巨大的小泡，导致了巨大小泡中的酶反应。

We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.