Analysis of cellular signal propagation of local stimulation by cell recognizable micro-sphere

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Numerous studies have shown that there are signal propagation between cells in a colony involved in the intercellular signaling. To analyze mechanism of the signaling propagation in the cell colony, we designed local stimulation technique using E-cadherin-immobilized micro-spheres or hydrogel, which gives local mechanical stimulation in a cell-sheet. We found that the local stimulation of E-cad-spheres to the periphery of the MDCK cell inhibit collective motion of the cell colony and inactivate ERK signaling, which propagated into the cell colony. Also, we found that local mechanical stimulation to the cell sheet induced ERK signaling to cells in a non-stimulated section suggested mechanical balance involved in actin network is one the transducer of signal propagation involved in collective cellular motion.