Pioneer study on the protective effect of ascorbic acid on barrier function of gastrointestinal tract

抗坏血酸对胃肠道屏障功能保护作用的开创性研究

• 批准号: 22658042
• 负责人: HORIO Fumihiko
• 金额: $ 2.38万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658042/
• 关键词: 栄養生化学; アスコルビン酸; ビタミンC; 腸管機能; 炎症; 急性期タンパク質

In ODS rats with a hereditary defect of ascorbic acid (vitamin C) biosynthesis, wehave demonstrated that ascorbic acid deficiency stimulate the hepatic expressions of acute phase protein genes, that were seen in inflammation. We hypothesized that the intestinal disorder provoked by ascorbic acid deficiency causes the inflow of endotoxin from gastrointesinal tract into portal vein, leading to the inflammatory responses in liver. By the analysis in this study, the endotoxin concentration in portal vein was significantly higher in the ascorbic acid-deficient group than that in the control group. Subsequetly, we confirmed whether the hepatic inflammatory responses occur or do not in ascorbic acid-deficient ODS rats treated with antibiotics. As a result, the hepatic inflammatory response was observed. From these results, we speculate that the inflow of endotoxin into the portal vein and the other unknown disorder by ascorbic acid deficiency cause the inflammatory responses in liver. However, this study demonstrated for the first time that ascorbic acid deficiency induce the disfunction of gastrointestinal tract and endotoxemia in ODS rats.

在抗坏血酸（维生素C）生物合成遗传缺陷的ODS大鼠中，我们已经证明抗坏血酸缺乏刺激肝脏急性期蛋白基因的表达，这在炎症中是可见的。我们假设由抗坏血酸缺乏引起的肠道疾病导致内毒素从胃肠道流入门静脉，导致肝脏炎症反应。本研究分析发现，抗坏血酸缺乏组门静脉内毒素浓度明显高于对照组。随后，我们证实了抗坏血酸缺乏的ODS大鼠在接受抗生素治疗后是否会发生肝脏炎症反应。结果，观察肝脏炎症反应。根据这些结果，我们推测内毒素流入门静脉以及其他未知的抗坏血酸缺乏引起的肝脏炎症反应。然而，本研究首次证实了抗坏血酸缺乏可引起ODS大鼠胃肠道功能障碍和内毒素血症。

项目成果

アスコルビン酸欠乏により惹起される肝臓の炎症様変化の機構解析

抗坏血酸缺乏引起肝脏炎症变化的机制分析

• 发表时间: 2011
• 作者: 三浦奈津子;星長夕貴子;小林美里;村井篤嗣;伊藤弘幸;森田達也;堀尾文彦
• 通讯作者: 堀尾文彦

ODSラットにおける、アスコルビン酸欠乏により惹起される肝臓の炎症様変化の解析

抗坏血酸缺乏引起ODS大鼠肝脏炎症变化分析

• 发表时间: 2011
• 作者: 三浦奈津子;星長夕貴子;小林美里;村井篤嗣;堀尾文彦
• 通讯作者: 堀尾文彦

Coffee and Caffeine Improve Insulin Sensitivity and Glucose Tolerance in C57BL/6J Mice Fed a High-Fat Diet

• DOI: 10.1271/bbb.110452
• 发表时间: 2011-12-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Matsuda, Yuji;Kobayashi, Misato;Horio, Fumihiko
• 通讯作者: Horio, Fumihiko

Genetic analysis of abdominal fat distribution in SM/J and A/Jmice.

SM/J 和 A/J 小鼠腹部脂肪分布的遗传分析。

• 发表时间: 2010
• 期刊: J Lipid Res.
• 作者: Kobayashi M;Ohno T;et al.
• 通讯作者: et al.