Essential role of poly(ADP-ribosyl)ation in maintenance of spermatogonial stem cells

聚（ADP-核糖基）化在维持精原干细胞中的重要作用

• 批准号: 23590097
• 负责人: TAKEHASHI MASANORI
• 金额: $ 3.41万
• 依托单位: Osaka Ohtani University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590097/
• 关键词: 精子幹細胞; ポリ(ADP-リボース); 多能性幹細胞; 発生・分化; 再生医療; ポリ(ADP-リボース）; ポリ(ＡＤＰ-リボース)

Spermatogonial stem cells (SSCs) can proliferate in vitro in the presence of glial cell line-derived neurotrophic factor, a self-renewal factor for SSCs. These cultured SSCs are called germline stem (GS) cells. In previous study, we examined the effect of PARP inhibitor, inhibition of poly(ADP-ribosyl)ation, on GS cells. Inhibition of poly(ADP-ribosyl)ation suppressed cell proliferation of GS cells, and induced cell death. In order to identify the causative genes that is involved in the pathway leading to the suppression of cell proliferation, the gene expression and protein phosphorylation analyses were carried out. Some genes in p53 signaling pathway were up-regulated after addition of PARP inhibitors. We then investigated activation of p53 by detection of its phosphorylation site. The phosphorylation was increased after addition of PARP inhibitors. These results suggest that poly(ADP-ribosyl)ation regulates activity of p53 and thus p53 signaling pathway in GS cells.

精原干细胞(SSCs)可以在胶质细胞系衍生神经营养因子(SSCs的自我更新因子)的存在下在体外增殖。这些培养的干细胞被称为生殖系干细胞(GS)。在先前的研究中，我们检测了PARP抑制剂，即抑制聚(ADP-核糖)，对GS细胞的影响。抑制聚腺苷二磷酸核糖基化抑制GS细胞增殖，诱导细胞死亡。为了找出参与抑制细胞增殖途径的致病基因，进行了基因表达和蛋白磷酸化分析。加入PARP抑制剂后，P53信号通路中的一些基因表达上调。然后，我们通过检测其磷酸化位点来研究P53的激活。加入PARP抑制剂后，磷酸化程度增加。这些结果表明，聚腺苷二磷酸核糖作用调节了P53的活性，从而调节了GS细胞中的P53信号通路。

项目成果

Mammalian Selenocysteine Lyase Is Involved in Selenoprotein Biosynthesis

• DOI: 10.3177/jnsv.57.298
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
• 影响因子: 1.6
• 作者: Kurokawa, Suguru;Takehashi, Masanori;Esaki, Nobuyoshi
• 通讯作者: Esaki, Nobuyoshi

Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in Mice

小鼠睾丸干细胞与体细胞和胚胎干细胞的杂交

• DOI: 10.1095/biolreprod.112.098988
• 发表时间: 2012
• 期刊: Biol. Reprod
• 作者: Takehashi;M.;Tada;M.;Kanatsu-Shinohara;M.;Morimoto;H.;Kazuki;Y.;Oshimura;M.;Tada;T. and Shinohara;T
• 通讯作者: T

ポリ(ADP-リボシル)化の制御が精子幹細胞に与える影響

控制聚（ADP-核糖基）化对精子干细胞的影响

• 发表时间: 2014
• 作者: 小野友久;他
• 通讯作者: 他

Inhibition of poly(ADP-ribose) polymerase-1 attenuates the toxicity of carbon tetrachloride

抑制聚（ADP-核糖）聚合酶-1 可减轻四氯化碳的毒性

• 发表时间: 2011
• 期刊: J Enzyme Inhib Med Chem
• 作者: Banasik M;Stedeford T;Strosznajder RP;Takehashi M;Tanaka S;Ueda K
• 通讯作者: Ueda K

PARP-1阻害剤によるp21を介した細胞周期制御

PARP-1 抑制剂介导的 p21 介导的细胞周期控制

• 作者: H. Sakurai;F.Ito;奥田明子
• 通讯作者: 奥田明子