Analysis of function of glucocorticoid receptor expressed in NPY neurons

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Body weight and white adipose tissues weight were significantly less in AgRP neuron-specific knockout of glucocorticoid receptor mice compared to wild-type mice when they were subjected to a high fat diet. The expression levels of UCP1 mRNA in the brown adipose tissues were significantly higher in the knockout mice than in wild-type mice. On the other hand, thapsigargin, an ER stressor, increased phosphorylation of p65 and attenuated expression of AgRP and NPY mRNA stimulated by dexamethasone in hypothalamic cultures of wild-type mice. Furthermore, it is demonstrated that inhibitor of p65 cancelled the effects of thapsigargin on the AgRP and NPY mRNA expression stimulated by dexamethasone.