Elucidation of the role of signal crosstalk by reactive oxygen species and cell adhesion in nephrogenesis and CAKUT

输尿管芽分支和肾发生这两种肾脏发生的基本现象与先天性肾和尿路畸形(CAKUT)的发生发展密切相关。本研究检测了NADPH氧化酶及其产物--活性氧(ROS)在输尿管芽分枝和肾发生中的作用。抑制NADPH氧化酶和ROS的产生导致输尿管芽分枝和肾脏发育受阻，从而导致肾脏发育不良和发育不良。细胞行为(细胞增殖、迁移、凋亡)和细胞黏附相关分子和MAP激酶的磷酸化信号转导与该机制密切相关。因此，NADPH氧化酶产生的ROS在输尿管芽分枝和肾发生中起重要作用。

Two nephrogenic basic phenomena, ureteric bud branching and nephrogenesis, closely participate in the development of congenital anomalies of the kidney and urinary tract (CAKUT). In this study, NADPH oxidase and the product, reactive oxygen species (ROS), were detected in the area of ureteric bud branching and nephrogenesis. The inhibition of NADPH oxidase and ROS production resulted in the suppression of ureteric bud branching and nephrogenesis, which contributed to hypoplastic and dysplastic kidney. Cell behavior (cell proliferation, migration, apoptosis) and phosphorylation signal transduction by both of cell adhesion-related molecules and MAP kinase were closely associated with the mechanism. Thus, ROS produced by NADPH oxidase plays an important role in ureteric bud branching and nephrogenesis.