Identification of novel tumor antigen by using autologous-tumor specific immune responses in thoracic malignancies

利用胸部恶性肿瘤的自体肿瘤特异性免疫反应鉴定新型肿瘤抗原

• 批准号: 23592080
• 负责人: TAKENOYAMA Mitsuhiro
• 金额: $ 3.24万
• 依托单位: National Hospital Organization, Kyushu Cancer Center (2012-2013)University of Occupational and Environmental Health, Japan (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592080/
• 关键词: 呼吸器悪性腫瘍; 腫瘍抗原; CTL; 特異的免疫応答

In order to developing immunotherapy as new modalities against thoracic malignancies with poor prognosis, it is important to analyze autologous tumor-specific immune responses. In this study, we induced several CTLs from patients with lung cancer, which are HLA-A2 or HLA-A24-restricted. Among them, CTL Clone 1 recognized the autologous large cell carcinoma cell line as well as allogenic lung cancer cell lines including adenocarcinoma and squamous cell carcinoma of the lung in an HLA-A24-restricted manner. cDNA clone coding antigen recognized by the CTL was isolated by cDNA expression cloning using cDNA library made from autologous tumor cells. These approaches will help us to develop new modalities for diagnosis and therapy for thoracic malignancies.

为了开发针对预后较差的胸部恶性肿瘤的新的免疫治疗手段，分析自体肿瘤特异性免疫反应具有重要意义。在本研究中，我们从肺癌患者中诱导了几种人类白细胞抗原A2或人类白细胞抗原A24限制性的CTL。其中，CTL Clone 1对包括肺腺癌和肺鳞癌在内的自体大细胞癌细胞系和同种异体肺癌细胞系均具有人类白细胞抗原A24限制性识别能力。用自体肿瘤细胞的cDNA文库进行表达克隆，获得CTL识别的编码抗原的cDNA克隆。这些方法将帮助我们为胸部恶性肿瘤的诊断和治疗开发新的模式。

项目成果

Prognostic implications of human leukocyte antigen class I expression in patients who underwent surgical resection for non-small-cell lung cancer

• DOI: 10.1016/j.jss.2012.07.029
• 发表时间: 2013-05-15
• 期刊: JOURNAL OF SURGICAL RESEARCH
• 影响因子: 2.2
• 作者: Hanagiri, Takeshi;Shigematsu, Yoshiki;Tanaka, Fumihiro
• 通讯作者: Tanaka, Fumihiro