Identification of novel tumor antigen by using autologous-tumor specific immune responses in thoracic malignancies

为了将免疫治疗作为治疗预后不良的胸部恶性肿瘤的新方式，分析自身肿瘤特异性免疫反应是很重要的。在本研究中，我们从肺癌患者中诱导了几种受HLA-A2或hla - a24限制的ctl。其中，CTL Clone 1以hla - a24限制的方式识别自体大细胞癌细胞系以及肺腺癌和鳞状细胞癌等异体肺癌细胞系。利用自体肿瘤细胞cDNA文库进行cDNA表达克隆，分离得到CTL识别的cDNA克隆编码抗原。这些方法将有助于我们开发新的模式诊断和治疗胸部恶性肿瘤。

In order to developing immunotherapy as new modalities against thoracic malignancies with poor prognosis, it is important to analyze autologous tumor-specific immune responses. In this study, we induced several CTLs from patients with lung cancer, which are HLA-A2 or HLA-A24-restricted. Among them, CTL Clone 1 recognized the autologous large cell carcinoma cell line as well as allogenic lung cancer cell lines including adenocarcinoma and squamous cell carcinoma of the lung in an HLA-A24-restricted manner. cDNA clone coding antigen recognized by the CTL was isolated by cDNA expression cloning using cDNA library made from autologous tumor cells. These approaches will help us to develop new modalities for diagnosis and therapy for thoracic malignancies.