Identification of stromal cells that have suppressive role againstpancreatic cancer cells and development of new anticancer therapy

本研究的目的是确定抑制胰腺癌细胞的基质细胞亚群。分析人胰腺导管腺癌(PDAC)来源的胰腺星状细胞(PSCs)的表面标志，鉴定CD271 PSCs。定量RT-PCR分析显示，PSCs与胰腺癌细胞共培养后CD271mRNA表达上调。而CD271mRNA的表达水平在短暂升高后下降。此外，PSCs通过Matrigel向胰腺癌细胞迁移时CD271mRNA表达降低，PDAC免疫组织化学显示间质CD271高表达与预后良好相关。这些发现提示CD271 PSCs出现在胰腺癌发生的早期阶段，并通过肿瘤-间质相互作用对胰腺癌细胞具有抑制作用。

The aim of the present study was to identify subpopulation of stromal cells that suppresspancreatic cancer cells. We analyzed surface marker of pancreatic stellate cells(PSCs) derived from human pancreatic ductal adenocarcinoma(PDAC), and characterized CD271+ PSCs.Quantitative RT-PCR analyses revealed that CD271 mRNA expression was increased in PSCs after coculture with pancreatic cancer cells. However, the level of CD271 mRNA expressionsubsequently decreased after the transient increase. Furthermore, CD271 mRNA expression was decreased in PSCs migrating toward pancreatic cancer cells through Matrigel.Immunohistochemistry of PDAC revealed stromal CD271 high expression was associated with a good prognosis. These findings suggest that CD271+ PSCs appear at the early stage ofpancreatic carcinogenesis and have suppressive role against pancreatic cancer cells through cancer-stromal interaction