Association between metabolic syndrome and periodontitis through adipocytokine in GCF

GCF中脂肪细胞因子与代谢综合征和牙周炎之间的关联

• 批准号: 23792471
• 负责人: SHIMADA Yasuko
• 金额: $ 2.75万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792471/
• 关键词: 慢性歯周炎; 歯肉溝滲出液; 歯肉溝浸出液

Eleven patients with generalized chronic periodontitis were enrolled. Forty biomarkers in GCF were examined using a multiplex bead immunoassay. Porphyromonas gingivalis (P. gingivalis) from the diseased sites was quantified by real-time polymerase chain reaction. Interleukin (IL)-1beta and interferon-inducible protein (IP)-10 levels were significantly higher in BOP-positive diseased sites than BOP-negative diseased sites after adjustment for multiple comparisons. In addition, the levels of IL-1beta in GCF were found to be strongly correlated with the P. gingivalis ratio. IL-1beta levels in GCF correlate with the presence of P. gingivalis in subgingival plaque. Multiplex bead assays can be useful in GCF studies. These findings can help in identifying new diagnostic methods in the diagnosis of periodontal disease.

11 名患有全身性慢性牙周炎的患者被纳入研究。使用多重珠免疫测定法检查了 GCF 中的 40 种生物标志物。通过实时聚合酶链式反应对患病部位的牙龈卟啉单胞菌（P.gingivalis）进行定量。多重比较调整后，BOP阳性病变部位的白细胞介素（IL）-1β和干扰素诱导蛋白（IP）-10水平显着高于BOP阴性病变部位。此外，GCF 中 IL-1β 的水平与牙龈卟啉单胞菌比率密切相关。 GCF 中的 IL-1β 水平与龈下菌斑中牙龈卟啉单胞菌的存在相关。多重微珠测定可用于 GCF 研究。这些发现有助于确定牙周病诊断的新诊断方法。

项目成果

歯肉溝滲出液中のバイオマーカーと歯周炎罹患部位の関連

龈沟液中生物标志物与牙周炎影响区域之间的关系

• 作者: 島田靖子;杉田典子;吉江弘正
• 通讯作者: 吉江弘正

Profiling biomakersin gingival crevicular fluid using multiplex bead immunoassay

使用多重珠免疫分析分析龈沟液中的生物标志物

• 作者: Yasuko Shimada;Noriko Sugita;Hiromasa Yoshie
• 通讯作者: Hiromasa Yoshie

Profiling biomarkers in gingival crevicular fluid using multiplex bead immunoassay

• DOI: 10.1016/j.archoralbio.2012.11.012
• 发表时间: 2013-06-01
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Shimada, Yasuko;Tabeta, Koichi;Yoshie, Hiromasa
• 通讯作者: Yoshie, Hiromasa

Profiling biomarkers in gingival crevicular fluid using multiplex bead immunoassay Yasuko Shimada

使用多重微珠免疫分析法分析龈沟液中的生物标志物 Yasuko Shimada

• 期刊: Archives of Oral Biology
• 影响因子: 3
• 作者: Koichi Tabeta;Noriko Sugita and Hiromasa Yoshie
• 通讯作者: Noriko Sugita and Hiromasa Yoshie