Why are male flower-specific genes of Cryptomeria japonica not expressed in callus?

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We tried to clarify the association between gene expression and methylation by analyzing the degree of methylation of the Muka14 promoter using bisulfite sequencing. We found that CpG sequences were methylated more than 1 kb upstream of the translation start point of the Muka14 gene, but were unmethylated downstream. The region required for male flower-specific expression of Muka14 is consistent with the distribution of unmethylated CpG. Our results suggest that the essential domains for the expression of target genes can be predicted using the methylation of promoters as an index.