Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis

未培养的自体脂肪来源的再生细胞在牵引成骨过程中促进骨形成

• 批准号: 24791529
• 负责人: WATANABE Koji
• 金额: $ 1.66万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791529/
• 关键词: 自己脂肪由来幹細胞; 体性幹細胞; 骨再生; 骨延長; 骨成熟促進

The question of this study was whether uncultured adipose-derived regenerative cells(ADRCs), which can easily be isolated from autologous adipose tissue and contain several types of stem and regenerative cells, promote bone formation in distraction osteogenesis. Sixty rats were randomly assigned to 3 groups. Physiological saline (control group), Type I collagen gel, or a mixture of ADRC and Type I collagen gel (ADRC group) was injected into the distracted callus immediately after distraction termination.The bone density of the distracted callus in the ADRC group increased by 46% compared with the control group at 6 weeks after injection. The fracture strength in the ADRC group increased by 66% compared with the control group at 6 weeks after injection. Real-time PCR of the distracted callus from the ADRC group had higher levels of BMP-2, VEGF A, and SDF-1. Cell labeling in the newly formed bone showed the ADRCs differentiated into osseous tissue at 3 weeks after injection.

本研究的问题是，未培养的脂肪来源的再生细胞（ADRC），它可以很容易地从自体脂肪组织中分离出来，并含有几种类型的干细胞和再生细胞，促进骨形成牵引成骨。将60只大鼠随机分为3组。牵张终止后立即将生理盐水（对照组）、I型胶原凝胶或ADRC与I型胶原凝胶的混合物（ADRC组）注入牵张骨痂内，6周时ADRC组牵张骨痂的骨密度较对照组增加46%。注射后6周，ADRC组的骨折强度比对照组增加了66%。ADRC组牵张骨痂的实时PCR显示BMP-2、VEGF A和SDF-1水平较高。新骨组织细胞标记显示，注射后3周，ADRCs分化为骨组织。

项目成果

Taylor Spatial Frameの矯正精度―脛骨変形矯正症例での評価―

泰勒空间框架的矫正精度 - 胫骨畸形矫正案例的评估 -

• 发表时间: 2012
• 期刊: 臨整外
• 作者: 渡邊孝治;松原秀憲;野村一世;土屋弘行
• 通讯作者: 土屋弘行

下肢長管骨感染性および非感染性偽関節に対するヨード担持チタンプレートによる治療

含碘钛板治疗感染性和非感染性下肢长骨假关节

• 发表时间: 2012
• 作者: 渡邊孝治;松原秀憲;野村一世;土屋弘行
• 通讯作者: 土屋弘行

Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis in Rats

• DOI: 10.1007/s11999-014-3608-8
• 发表时间: 2014-12-01
• 期刊: CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
• 影响因子: 4.2
• 作者: Nomura, Issei;Watanabe, Koji;Tsuchiya, Hiroyuki
• 通讯作者: Tsuchiya, Hiroyuki

A surgical procedure for fracture healing with superior soft tissue preservation

骨折愈合并保留良好软组织的外科手术

• 发表时间: 2012
• 作者: Watanabe K;Matsubara H;Nomura I;Tsuchiya H.
• 通讯作者: Tsuchiya H.

Adipose-derived regenerative cells promote bone formation on distraction osteogenesis in rats

脂肪来源的再生细胞促进大鼠牵张成骨的骨形成

• 发表时间: 2014
• 作者: I. Nomura;K. Watanabe;H. Matsubara;K. Hayashi;H. Tsuchiya
• 通讯作者: H. Tsuchiya