Assessment of biological effect of low-dose radiation by phosphorylated H2AX in vivo

磷酸化 H2AX 体内低剂量辐射的生物学效应评估

• 批准号: 24700955
• 负责人: NAKAMURA Asako
• 金额: $ 4.16万
• 依托单位: Ibaraki University (2013)Osaka Medical College (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700955/
• 关键词: 低線量放射線; DNA損傷; 発がん

Radiation induced-DNA double strand break (DSB) is one major initial cause of genomic instability leading to cancer. The creation of a DNA DSB in eukaryotic cells is generally accompanied by the formation of hundreds of phosphorylated H2AX (gamma-H2AX) molecules in the chromatin flanking the DSB site. Antibodies to gamma-H2AX allow the visualization of a "focus" at the DSB site. Since it has been demonstrated that the numbers of gamma-H2AX foci correlate directly to the numbers of DNA DSB, the detection of gamma-H2AX is an attractive candidate for monitoring DNA DSB levels. Here, to assess biological effect by radiation exposure after the accident, we performed quantitation of gamma-H2AX foci as a maker of DNA DSB in bovine lymphocytes from Fukushima evacuated area. Although majority of lymphocytes are negative of gamma-H2AX foci, immunostaining data clearly showed an increase of population of gamma-H2AX foci positive cells from Fukushima evacuated area.

辐射诱导的dna双链断裂（DSB）是导致癌症的基因组不稳定的主要初始原因之一。真核细胞中DNA DSB的形成通常伴随着在DSB位点两侧染色质中形成数百个磷酸化的H2AX （γ -H2AX）分子。针对γ - h2ax的抗体可以使DSB位点的“焦点”可视化。由于已经证明γ - h2ax焦点的数量与DNA DSB的数量直接相关，因此检测γ - h2ax是监测DNA DSB水平的有吸引力的候选物。在这里，为了评估事故后辐射暴露的生物效应，我们对福岛疏散区牛淋巴细胞中γ - h2ax焦点作为DNA DSB的制造者进行了定量。虽然大多数淋巴细胞γ - h2ax灶呈阴性，但免疫染色数据清楚地显示福岛疏散区γ - h2ax灶阳性细胞数量增加。

项目成果

Monitoring low-dose radiation induced-DNA damage levels in vivo

监测体内低剂量辐射引起的 DNA 损伤水平

• 发表时间: 2013
• 作者: NAKAMURA;Masatoshi SUZUKI;Manabu FUKUMOTO
• 通讯作者: Manabu FUKUMOTO

Methods Mol Biol

分子生物学方法

• 发表时间: 2013
• 作者: Ishii;K. Amagai;M.
• 通讯作者: M.

高感度DNA 損傷マーカーを用いた生体応答の評価

使用高度灵敏的 DNA 损伤标记物评估生物反应

• 发表时间: 2014
• 作者: Ivashkevich A;Redon CE;Nakamura AJ;Martin RF;Martin OA.;中村麻子;中村麻子
• 通讯作者: 中村麻子

Advances in Genet. Res.

基因进展。

• 发表时间: 2012
• 作者: Nakamura AJ;Parekh P;martin OA;Bonner WM and Redon CE.
• 通讯作者: Bonner WM and Redon CE.

The use of γ-H2AX assay for validation of novel radioprotector against both acute and chronic low-dose radiation exposure

使用 γ-H2AX 测定法验证新型放射防护剂对急性和慢性低剂量辐射暴露的影响

• 发表时间: 2014
• 作者: Asako J. Nakamura;Megumi Sasatani and Kenji Kamiya
• 通讯作者: Megumi Sasatani and Kenji Kamiya