Establishment of a xeno-free primary culture method for human colonic epithelial stem cells

人结肠上皮干细胞无异源原代培养方法的建立

• 批准号: 25860524
• 负责人: MIZUTANI Tomohiro
• 金额: $ 2.66万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25860524/
• 关键词: 腸管上皮培養; 低分子化合物; GMP規格; 再生医療; 下部消化管学（小腸、大腸）; 人工ペプチド

项目成果

Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine.

• DOI: 10.7717/peerj.370
• 发表时间: 2014
• 期刊: PeerJ
• 影响因子: 2.7
• 作者: Shimizu H;Okamoto R;Ito G;Fujii S;Nakata T;Suzuki K;Murano T;Mizutani T;Tsuchiya K;Nakamura T;Hozumi K;Watanabe M
• 通讯作者: Watanabe M

Fluorescent labelling of intestinal epithelial cells reveals independent long-lived intestinal stem cells in a crypt.

肠上皮细胞的荧光标记揭示了隐窝中独立的长寿命肠干细胞。

• DOI: 10.1016/j.bbrc.2014.10.091
• 发表时间: 2014
• 期刊: Biochem Biophys Res Commun
• 作者: N. Horita;K. Tsuchiya;R. Hayashi;K. Fukushima;S. Hibiya;M. Fukuda;Y. Kano;T. Mizutani;Y. Nemoto;S. Yui;R. Okamoto;T. Nakamura;M. Watanabe
• 通讯作者: M. Watanabe

Hes1 promotes the IL-22-mediated antimicrobial response by enhancing STAT3-dependent transcription in human intestinal epithelial cells

• DOI: 10.1016/j.bbrc.2013.12.061
• 发表时间: 2014-01-17
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Murano, Tatsuro;Okamoto, Ryuichi;Watanabe, Mamoru
• 通讯作者: Watanabe, Mamoru

Successful Engraftment of Cultured Small Intestinal Epithelial Stem Cells onto Damaged Colonic Mucosa by Heterotopic Transplantation

通过异位移植成功地将培养的小肠上皮干细胞移植到受损的结肠粘膜上

• 发表时间: 2014
• 作者: Fukuda M;Mizutani T;Mochizuki W;Matsumoto T;Nozaki K;Ichinose S;Watanabe M;Nakamura T
• 通讯作者: Nakamura T

Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells.

• DOI: 10.1371/journal.pone.0079693
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ito G;Okamoto R;Murano T;Shimizu H;Fujii S;Nakata T;Mizutani T;Yui S;Akiyama-Morio J;Nemoto Y;Okada E;Araki A;Ohtsuka K;Tsuchiya K;Nakamura T;Watanabe M
• 通讯作者: Watanabe M