Expression of vascular endothelial cell growth factor and regeneration of hepatic endothelial cells during liver regeneration.

大鼠肝部分切除后，残肝在一周内迅速再生。为探讨血管生成生长因子在肝内皮细胞再生中的作用，本实验利用低分子量血管内皮生长因子(VEGF121)可以进入血液并参与再生肝血管内皮细胞的作用，检测了血管内皮细胞生长因子(VEGF-A和VEGF-B)和碱性成纤维细胞生长因子(BeGf)在再生肝和其他器官中的表达，发现在再生肝中，VEGF-A的表达在肝部分切除后即刻升高，并维持高水平表达。碱性成纤维细胞生长因子的表达呈增加趋势，而血管内皮生长因子-B的表达无明显变化。在再生肝中，未观察到血管内皮生长因子-A和血管内皮生长因子-B的剪接变异体的变化。肺和心脏持续高水平表达VEGF-A，前者表达VEGF121，而后者不表达。在肝脏再生过程中，S的…没有明显变化血管内皮生长因子-A在两个脏器中的表达更明显。脾、肾组织中VEGF-A表达增加近一倍，脾组织中检测到VEGFl2 I。VEGF-B在肺、心、脾和小肠中的表达增加，而在肝、肾中的表达没有变化。这些结果提示，肝组织中表达的血管内皮生长因子-A和碱性成纤维细胞生长因子可能参与了非实质细胞的再生，肺、脾产生的VEGFl2I和来自肺、心、脾和小肠的血管内皮生长因子-B也可能参与了再生。这些因子表达增加的机制仍不清楚。然而，我们发现肌肉脂肪组织中的血管内皮生长因子-A和碱性成纤维细胞生长因子的表达受交感神经的控制。还应该注意的是，交感神经系统在肝脏再生过程中被激活。因此，血管内皮生长因子-A、血管内皮生长因子-B和碱性成纤维细胞生长因子的表达可能受到肌肉脂肪组织等交感神经系统的调节。较少

After partial hepatectomy in the rat, the remnant liver rapidly regenerates within a week. To evaluate the involvement of angiogenic growth factor in regeneration of hepatic endothelial cells, I determined the expression of vascular endothelial cell growth factor (VEGF-A and VEGF-B) and basic fibroblast growth factor (beGf) in the regenerating liver and other organs since low molecular weight VEGF (VEGF 121) could enter the blood and might be involved in vascular endothelial cells in the regenerating liver, In the liver, VEGF-A expression was increased immediately after partial hepatectomy, and sustained high level of the expression. The expression of bFGF was tend to increase, but the expression of VEGF-B was not changed. No change was observed in splicing variants of VEGF-A and VEGF-B in the regenerating liver. Lung and heart persistently expressed VEGF-A at high levels, and the former, but not the latter, expressed VEGF 121. During the regeneration of liver, no apparent change was s … More een in the expression of VEGF-A in the both organs. The VEGF-A expression in spleen and kidney was almost doubled, and VEGFl2 I was detected in spleen. The expression of VEGF-B was increased in lung, heart, spleen and small intestine, although it was not changed in liver and kidney. These results suggest that hepatic expression of VEGF-A and bFGF in the regenerating liver might contribute the regeneration of non-parenchymal cells, and that VEGFl2I produced in lung and spleen and VEGF-B from lung, heart, spleen and small intestine might also be involved in the regeneration. The mechanism by which expression of these factors increases is still obscure. However, we found the expression of VEGF-A and bFGF in brawn adipose tissue was controlled by the sympathetic nerve. It should be also noted that the sympathetic nerve systems are activated during the liver regeneration. Therefore, the expression of VEGF-A, VEGF-B and bFGF might be regulated by the sympathetic nerve systems like brawn adipose tissue. Less