Biomaterial having active sites of collagen-binding adhesion molecules (CMP and RGD-CAP)

化学机械抛光涂层增强了各种软骨细胞和成纤维细胞的黏附和铺展。11型胶原协同增强了cMP对细胞铺展的作用。我们还发现，1251-CMP利用针对各种整合素亚基的单抗，选择性地直接与α-β整合素结合。此外，我们还克隆了兔Matrilin1和兔matrilin3的cDNA，以确定刺激细胞黏附的活性部位。RGD-CAP涂层还增强了各种软骨细胞和成纤维细胞的黏附和铺展。C末端附近的RGD序列对黏附活性不是必需的。对不同整合素亚基的单抗研究表明，RGD-CAP是一种不与RGD基序结合的新的α1β1整合素配体。我们已经证明，RGD-CAP分子中至少有两个重复结构是刺激细胞黏附所必需的。这些发现将有助于将CMP和RGD-CAP及其片段作为生物材料在组织工程中的临床应用。

The CMP coating enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The effect of CMP on the cell spreading was synergistically increased by type 11 collagen. We also showed that 1251-CMP selectively binds to alphalbetal integrin directly using monoclonal antibodies to various integrin subunits. In addition, we cloned cDNAS for rabbit matrilin1 (CMP) and rabbit matrilin3 to identify active sites for the stimulation of cell adhesion.The RGD-CAP coating also enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The RGD sequence near the C-terminal was not essential for the adhesion activity. Studies with monoclonal antibodies to various integrin subunits showed that RGD-CAP is a novel ligand for alpha1beta1 integrin that does not bind to the RGD motif. We have shown that at least two repeated structures within the RGD-CAP molecule are required for the stimulation of cell adhesion. These findings will be useful for clinical application of CMP and RGD-CAP, as well as their fragments, as biomaterials in tissue engineering.