Biomaterial having active sites of collagen-binding adhesion molecules (CMP and RGD-CAP)

CMP涂层增强了各种软骨细胞和成纤维细胞的粘附和扩散。CMP对细胞扩散的影响与11型胶原有协同作用。我们还发现，1251-CMP通过针对各种整合素亚基的单克隆抗体选择性地直接结合到阿尔法统合素上。此外，我们克隆了兔matrilin1 （CMP）和兔matrilin3的cdna，以确定刺激细胞粘附的活性位点。RGD-CAP涂层还增强了各种软骨细胞和成纤维细胞的粘附和扩散。靠近c末端的RGD序列对粘附活性不是必需的。针对各种整合素亚基的单克隆抗体的研究表明，RGD- cap是一种不结合RGD基序的alpha1beta1整合素的新型配体。我们已经证明，RGD-CAP分子中至少有两个重复的结构是刺激细胞粘附所必需的。这些发现将有助于CMP和RGD-CAP及其片段作为组织工程生物材料的临床应用。

The CMP coating enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The effect of CMP on the cell spreading was synergistically increased by type 11 collagen. We also showed that 1251-CMP selectively binds to alphalbetal integrin directly using monoclonal antibodies to various integrin subunits. In addition, we cloned cDNAS for rabbit matrilin1 (CMP) and rabbit matrilin3 to identify active sites for the stimulation of cell adhesion.The RGD-CAP coating also enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The RGD sequence near the C-terminal was not essential for the adhesion activity. Studies with monoclonal antibodies to various integrin subunits showed that RGD-CAP is a novel ligand for alpha1beta1 integrin that does not bind to the RGD motif. We have shown that at least two repeated structures within the RGD-CAP molecule are required for the stimulation of cell adhesion. These findings will be useful for clinical application of CMP and RGD-CAP, as well as their fragments, as biomaterials in tissue engineering.