Biomaterial having active sites of collagen-binding adhesion molecules (CMP and RGD-CAP)

CMP涂层增强了各种软骨细胞和成纤维细胞的粘附和铺展。CMP对细胞铺展的作用通过11型胶原协同增加。我们还表明，1251-CMP选择性地结合直接使用单克隆抗体的各种整合素亚基的前列腺整合素。此外，我们克隆了兔matrilin 1（CMP）和兔matrilin 3的cDNA，以确定其刺激细胞粘附的活性位点。RGD-CAP涂层还增强了各种软骨细胞和成纤维细胞的粘附和铺展。C端附近的RGD序列对粘附活性不是必需的。对不同整合素亚基的单克隆抗体的研究表明，RGD-CAP是不与RGD基序结合的α 1 β 1整合素的新型配体。我们已经表明，RGD-CAP分子内至少有两个重复的结构是刺激细胞粘附所必需的。这些结果将有助于CMP和RGD-CAP及其片段作为组织工程生物材料的临床应用。

The CMP coating enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The effect of CMP on the cell spreading was synergistically increased by type 11 collagen. We also showed that 1251-CMP selectively binds to alphalbetal integrin directly using monoclonal antibodies to various integrin subunits. In addition, we cloned cDNAS for rabbit matrilin1 (CMP) and rabbit matrilin3 to identify active sites for the stimulation of cell adhesion.The RGD-CAP coating also enhanced the adhesion and spreading of various chondrocytes and fibroblasts. The RGD sequence near the C-terminal was not essential for the adhesion activity. Studies with monoclonal antibodies to various integrin subunits showed that RGD-CAP is a novel ligand for alpha1beta1 integrin that does not bind to the RGD motif. We have shown that at least two repeated structures within the RGD-CAP molecule are required for the stimulation of cell adhesion. These findings will be useful for clinical application of CMP and RGD-CAP, as well as their fragments, as biomaterials in tissue engineering.