Bioartificial organs which promote self organ regeneration.

促进自身器官再生的生物人工器官。

• 批准号: 09357012
• 负责人: SHIMIZU Yasuhiko
• 金额: $ 14.46万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09357012/
• 关键词: regeneration of auto-tissue; extracellular matrix; enzyme extraction collagen; growth factor; stem cell; three-dimensional structure; 人工臓器; 再生医学; 人工血管; 人工食道; 人工気管; コラーゲン; 人工神経

The collagen which is a natural derived biopolymer material plays large role for differentiation, proliferation, repair, maintenance of the organism as a extracellular matrix. In our project, what kind of renewal process the cell of the organism took using this collagen, was clarified. The completely new bio-artificial organ that reproduces and induces self-organization and self organ by giving the scaffold of this collagen, is developed. Continuously artificial esophagus, artificial trachea, artificial blood vessel, artificial nerve experimentally were produced in last year, the experiment was mainly carried out in dogs. Artificial esophagus of 15cm length was tried in pleural cavity, the IVH management was carried out throughout for 2 months, and the regeneration of the gullet organization was observed. The self-organization entered the collagen implanted after 2 months, and it was proven that the tube made of fibrous tissue was formed. However, it rapidly contracted after the stent … More removal, and this tube is stenosed, the dog dies in this reason. It seemed to be the key technique that improves by preventing the stenosis after this stent evulsion. As for the improvement of the result by the artificial trachea, the trachea wall was improved, which was composed of collagen sponge which promoted the regeneration and differentiation of the self-organization, so as to prevent fistula. With this improvement, operative result was rapidly improved on Y-type bifurcation-type artificial treacheae since autumn, 1998. At present, the dog which succeeded in the bifurcational reconstruction has exceeded 10. The artificial blood vessel also was continuously subject of the experiment in which transplants XENO artificial blood vessel was designed from carotid artery of pigs and applied to an abdominal aorta of the dog. Blood vessel component cell of the pig was removed with detergent In removing, heparinized technique which gives antithrombogenesity has been established. The artificial nerve was studied namely the method for coupling the peripheral nerve in the collagen tube, and we tried the experiment which rapidly induce the regeneration of 8cm gap with a tube filled by the collagen fiber coated by laminin, and it succeeded in the regeneration. Less

胶原蛋白是一种天然衍生的生物聚合物材料，作为细胞外基质对生物体的分化、增殖、修复、维持起着重要的作用。在我们的项目中，使用这种胶原蛋白的有机体细胞经历了什么样的更新过程，被阐明了。一种全新的生物人造器官被开发出来，它通过给予这种胶原蛋白支架来复制和诱导自组织和自我器官。连续人工食道、人工气管、人工血管、人工神经已于去年陆续试制出来，实验主要在犬身上进行。胸腔内放置15 cm人工食道，连续2个月行IVH治疗，观察食道组织再生情况。植入2个月后，自组织进入胶原层，证明由纤维组织构成的管子已形成。然而，在支架…后，它迅速收缩再取下，如果这根管子狭窄，狗就会因此而死亡。这似乎是预防支架拔除后狭窄的关键技术。对于人工气管效果的改善，改善了气管壁，由胶原海绵组成，促进了自组织的再生和分化，从而防止了瘘的发生。自1998年秋季以来，Y型分叉型人工泪道手术效果得到迅速改善。目前，成功分叉重建的犬已超过10只。人工血管也连续进行了从猪的颈动脉设计的Xeno人工血管移植到狗的腹主动脉的实验。用去污剂去除猪血管成分细胞，建立了具有抗血栓作用的肝素化技术。研究了人工神经与周围神经在胶原管中偶联的方法，并尝试了用包被层粘连蛋白的胶原纤维填充胶原管快速诱导8 cm缝隙再生的实验，成功地进行了再生。较少

项目成果

Americo K Kitahara: "Facial nerve repair using collagen nerve conduit in cat"Scandinavian Jounal of Plastic and Reconstructive Surgery and Hand Surgery. 33. 187-193 (1999)

Americo K Kitahara：“使用猫的胶原神经导管进行面部神经修复”斯堪的纳维亚整形外科和手外科杂志。

清水慶彦: "縦隔臓器の再建・再生"現代医学. 31(12). 125-129 (1999)

清水义彦：“纵隔器官的重建和再生”现代医学31（12）（1999）。

Yasuhiko Shimizu: "Regeneration of peripheral nerve."Tissue Engineering.. 186-197 (1999)

Yasuhiko Shimizu：“周围神经的再生。”组织工程.. 186-197 (1999)

Y.Yamamoto: "Intrathoracic esophageal replacement in the dog with the use of an artificial esophagus composed of a collagen sponge with a double-layered silicone tube"Journal of Thoracic Cardiovascular Surgery. 118(2). 276-286 (1999)

Y.Yamamoto：“使用由胶原海绵和双层硅胶管组成的人工食管对狗进行胸腔内食管置换”《胸心血管外科杂志》。

中村達雄: "軟組織(膜・気管・食道)の再生"Pharma Medica. 18(1). 75-80 (2000)

Tatsuo Nakamura：“软组织再生（膜、气管、食道）”Pharma Medica 18(1) (2000)。