Bioartificial organs which promote self organ regeneration.

胶原蛋白是一种天然衍生的生物高分子材料，作为细胞外基质，在机体的分化、增殖、修复、维持等方面发挥着重要作用。在我们的项目中，生物体的细胞使用这种胶原蛋白进行了什么样的更新过程，得到了澄清。以胶原蛋白为支架，再生诱导自组织和自器官的全新生物人工器官被开发出来。去年连续进行了人工食管、人工气管、人工血管、人工神经的实验，实验主要是在狗身上进行的。在胸腔内尝试长15cm的人工食管，IVH处理持续2个月，观察食管组织再生情况。2个月后，自组织进入植入的胶原蛋白中，证明纤维组织制成的管已经形成。但是，在支架置入后，它迅速收缩……更多的移除，并且这条管子是狭窄的，狗因此死亡。预防支架脱落后血管狭窄似乎是改善血管功能的关键技术。人工气管改善气管管壁，气管管壁由胶原海绵组成，促进自组织的再生和分化，防止瘘的发生。自1998年秋季以来，y型分岔型人工气管手术效果迅速提高。目前，成功进行分岔重建的犬已超过10只。人工血管也是实验的持续对象，从猪颈动脉设计移植XENO人工血管，应用于犬腹主动脉。用去污剂去除猪血管成分细胞，建立了抗血栓形成的肝素化去除技术。我们研究了人工神经即外周神经在胶原管内的耦合方法，并尝试了用层粘连蛋白包被的胶原纤维填充管内快速诱导8cm间隙再生的实验，并成功实现了再生。少

The collagen which is a natural derived biopolymer material plays large role for differentiation, proliferation, repair, maintenance of the organism as a extracellular matrix. In our project, what kind of renewal process the cell of the organism took using this collagen, was clarified. The completely new bio-artificial organ that reproduces and induces self-organization and self organ by giving the scaffold of this collagen, is developed. Continuously artificial esophagus, artificial trachea, artificial blood vessel, artificial nerve experimentally were produced in last year, the experiment was mainly carried out in dogs. Artificial esophagus of 15cm length was tried in pleural cavity, the IVH management was carried out throughout for 2 months, and the regeneration of the gullet organization was observed. The self-organization entered the collagen implanted after 2 months, and it was proven that the tube made of fibrous tissue was formed. However, it rapidly contracted after the stent … More removal, and this tube is stenosed, the dog dies in this reason. It seemed to be the key technique that improves by preventing the stenosis after this stent evulsion. As for the improvement of the result by the artificial trachea, the trachea wall was improved, which was composed of collagen sponge which promoted the regeneration and differentiation of the self-organization, so as to prevent fistula. With this improvement, operative result was rapidly improved on Y-type bifurcation-type artificial treacheae since autumn, 1998. At present, the dog which succeeded in the bifurcational reconstruction has exceeded 10. The artificial blood vessel also was continuously subject of the experiment in which transplants XENO artificial blood vessel was designed from carotid artery of pigs and applied to an abdominal aorta of the dog. Blood vessel component cell of the pig was removed with detergent In removing, heparinized technique which gives antithrombogenesity has been established. The artificial nerve was studied namely the method for coupling the peripheral nerve in the collagen tube, and we tried the experiment which rapidly induce the regeneration of 8cm gap with a tube filled by the collagen fiber coated by laminin, and it succeeded in the regeneration. Less