Bioartificial organs which promote self organ regeneration.

胶原蛋白是一种天然来源的生物高分子材料，作为细胞外基质对生物体的分化、增殖、修复、维持起着重要作用。在我们的项目中，阐明了使用这种胶原蛋白的生物体细胞的更新过程。通过给予这种胶原蛋白的支架，开发出了全新的生物人工器官，该生物人工器官复制并诱导自组织和自器官。近年来连续实验性地研制了人工食管、人工气管、人工血管、人工神经，主要在犬体内进行实验。在胸腔内尝试植入15cm人工食管，进行IVH治疗2个月，观察食管组织再生情况。2个月后，自组织进入植入的胶原蛋白中，并证明形成了由纤维组织制成的管。然而，在植入支架后， ...更多信息 这是一个很大的问题，这是一个很大的问题，这是一个很大的问题。预防支架拔出后再狭窄是提高支架质量的关键技术。人工气管的效果改善方面，通过对气管壁的改良，使气管壁由胶原海绵组成，促进自组织的再生和分化，从而达到预防瘘的目的。通过这一改进，自1998年秋季以来，Y型分叉型人工引流管的手术效果迅速提高。目前，成功进行分叉重建的犬已超过10只。人造血管还连续地进行实验，其中移植XENO人造血管是从猪的颈动脉设计的，并应用于狗的腹主动脉。用去污剂去除猪的血管成分细胞，建立了肝素化的抗血栓形成技术。对人工神经即周围神经在胶原管中的耦合方法进行了研究，并尝试了用层粘连蛋白包被的胶原纤维填充的管快速诱导8cm间隙的再生实验，并获得了成功。少

The collagen which is a natural derived biopolymer material plays large role for differentiation, proliferation, repair, maintenance of the organism as a extracellular matrix. In our project, what kind of renewal process the cell of the organism took using this collagen, was clarified. The completely new bio-artificial organ that reproduces and induces self-organization and self organ by giving the scaffold of this collagen, is developed. Continuously artificial esophagus, artificial trachea, artificial blood vessel, artificial nerve experimentally were produced in last year, the experiment was mainly carried out in dogs. Artificial esophagus of 15cm length was tried in pleural cavity, the IVH management was carried out throughout for 2 months, and the regeneration of the gullet organization was observed. The self-organization entered the collagen implanted after 2 months, and it was proven that the tube made of fibrous tissue was formed. However, it rapidly contracted after the stent … More removal, and this tube is stenosed, the dog dies in this reason. It seemed to be the key technique that improves by preventing the stenosis after this stent evulsion. As for the improvement of the result by the artificial trachea, the trachea wall was improved, which was composed of collagen sponge which promoted the regeneration and differentiation of the self-organization, so as to prevent fistula. With this improvement, operative result was rapidly improved on Y-type bifurcation-type artificial treacheae since autumn, 1998. At present, the dog which succeeded in the bifurcational reconstruction has exceeded 10. The artificial blood vessel also was continuously subject of the experiment in which transplants XENO artificial blood vessel was designed from carotid artery of pigs and applied to an abdominal aorta of the dog. Blood vessel component cell of the pig was removed with detergent In removing, heparinized technique which gives antithrombogenesity has been established. The artificial nerve was studied namely the method for coupling the peripheral nerve in the collagen tube, and we tried the experiment which rapidly induce the regeneration of 8cm gap with a tube filled by the collagen fiber coated by laminin, and it succeeded in the regeneration. Less